نتایج جستجو برای: time qpcr
تعداد نتایج: 1899469 فیلتر نتایج به سال:
Quantitative real-time PCR is an important high-throughput method in the biomedical sciences. However, existing software has limitations in handling both relative and absolute quantification. We designed quantitative PCR data analysis and management system (qPCR-DAMS), a database tool based on Access 2003, to deal with such shortcomings by the addition of integrated mathematical procedures. qPC...
Reducing the time taken to run qPCR assays on today's qPCR cyclers is rather straightforward and requires no specialised reagents or instruments. As the first article in a new series of short technical reports, I demonstrate that it is possible to reduce significantly both denaturation temperatures and cycling times, whilst retaining sensitivity and specificity of the original qPCR conditions.
This paper assesses the quantitative resolution of qPCR using copy number variation (CNV) as a paradigm. An error model is developed for real-time qPCR data showing how the precision of CNV determination varies with the number of replicates. Using samples with varying numbers of X chromosomes, experimental data demonstrates that real-time qPCR can readily distinguish four copes from five copies...
We investigated Legionella contamination in bath water samples, collected from 68 bathing facilities in Japan, by culture, culture with amoebic co-culture, real-time quantitative PCR (qPCR), and real-time qPCR with amoebic co-culture. Using the conventional culture method, Legionella pneumophila was detected in 11 samples (11/68, 16.2%). Contrary to our expectation, the culture method with the ...
This study aimed to report histopathological features and serological outputs of the lung, heart and liver in a patient suffered from Coronavirus disease-2019 (COVID-19). A woman was admitted to the Razi Hospital, Rasht city, Iran with the symptoms of cough, dyspnea, fever and myalgia. She had also Parkinson’s disease (PD); she had no history of respiratory, cardiovascular, renal and gastrointe...
In recent years, increasing numbers of human campylobacteriosis cases caused by contaminated water have been reported. As the culture-based detection of Campylobacter is time consuming and can yield false-negative results, the suitability of a quantitative real-time PCR method in combination with an ethidium monoazide pretreatment of samples (EMA-qPCR) for the rapid, quantitative detection of v...
کلزا (brassica napus) گیاهی زراعی با ارزش اقتصادی قابل توجهی است که همواره تحمل آن نسبت به تنش ها مورد توجه بوده است. تنش سرما (دمای بین صفر تا 20 درجه سانتیگراد) و تنش انجماد (دمای کمتر از صفر درجه سانتیگراد) می تواند رشد و بازده گیاهان زراعی را تحت تأثیر قرار دهد و میزان این تأثیرپذیری بستگی به نوع تنش و میزان عملکرد ژنتیکی گیاه و مکانیسم مقاومت آن دارد. در بررسی حاضر، ارقام slmo46 و quantum ...
cholera is an infection of the small intestines caused by the bacterium v. cholerae. it is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. the major virulence factor produced by v. cholerae during infection is the cholera toxin. total mrna extraction and reverse transcription was performed for making ctxab cdna. relative real-time...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید