BACKGROUND Glucose metabolism links metabolic status to protein acetylation. However, it remains poorly understood to what extent do features of glucose metabolism contribute to protein acetylation and whether the process can be dynamically and quantitatively regulated by differing rates of glycolysis. RESULTS Here, we show that titratable rates of glycolysis with corresponding changes in the...

The effects of varying extracellular pH on the rates of uptake of titratable anions by human erythrocytes under conditions of constant intracellular pH have been determined for a series of highly related anions, the phosphate "analogs." These compounds are simply substituted phosphorus oxyacids, differing in the number and acidity of titratable protons: phosphate (HPO4(2-), pKa 6.8); phosphite ...

Probiotic beverages made from cereals become interesting in the recent food industries. In this contribution, a fermented teff-based probiotic beverage was produced using whole grain teff flour and co-culture strains of Lactiplantibacillus plantarum (LPA6) Lacticaseibacillus rhamnosus GG (LCGG). Then, effect 25 days refrigerated storage on cell viability (LPA6 LCGG), contents sugars, organic ac...

It has been known since the work of Mayer (1) in 1875 that the thick succulent leaves of many plants of the family Crassulaceae become enriched in titratable organic acids during the night and diminish in acid content when the leaves are illuminated. An analogous diurnal variation in acidity has been observed in a number of other succulents, and occasionally in species of different growth habit...

The interplay between biochemical characteristics and the generation of volatile compounds in 11 type II sourdough fermented by single strains lactic acid bacteria (LAB) was studied. Samples were collected at 0, 6, 9, 12, 15, 18 24h for analyses microbial growth, pH, titratable acidity CO2 production. During first 12h, LABs entered stationary phase, formation organic carboxyl acids, alcohols, e...

Titrations were made of yellow fever virus in stegomyia mosquitoes, using rhesus monkeys as test animals. It was found that: (a) The average mosquito immediately after engorging on highly infectious blood contained between 1 and 2 million lethal doses of virus. The titer of freshly ingested blood was as high as 1 billion lethal doses of virus per cubic centimeter. (b) During the fortnight succe...

To test the hypothesis that amino acid residues in band 3 with titratable positive charges play a role in the binding of anions to the outside-facing transport site, we measured the effects of changing external pH (pH(O)) on the dissociation constant for binding of external iodide to the transport site, K(O)(I). K(O)(I) increased with increasing pH(O), and a significant increase was seen even a...

Correct protonation of titratable groups in biomolecules is crucial for their accurate description by molecular dynamics simulations. In the context of constant pH simulations, an additional protonation degree of freedom is introduced for each titratable site, allowing the protonation state to change dynamically with changing structure or electrostatics. Here, we extend previous approaches for ...