In this work, the solubility of nicotine extracted from tobacco leaves, found in the north of Iran, in supercritical carbon dioxide has been measured. Also the effects of pressure, temperature, extraction dynamic time, and the organic co-Solvent on the amount of nicotine extracted from tobacco leaves have been investigated. It should be stressed that in order to reduce significantly the num...

Effects of tobacco genetically modified to express the protease inhibitor bovine spleen trypsin inhibitor (BSTI) were examined in laboratory assays against three earthworm and one collembolan species. BSTI is a serine protease inhibitor that can bind to the digestive trypsins of insects feeding on modified plants, resulting in reduced growth and survival. Protease inhibitors are active against ...

Tobacco mosaic virus (TMV) is one of the most devastating viruses to crops, which can cause severe production loss and affect the quality of products. In this study, we have proposed a novel approach to discriminate TMV-infected tobacco based on laser-induced breakdown spectroscopy (LIBS). Two different kinds of tobacco samples (fresh leaves and dried leaf pellets) were collected for spectral a...

The chemical and physical characteristics of Cd-binding peptides isolated from tobacco (Nicotiana tabacum) leaves and suspension-cultured tobacco cells were determined and compared with properties of rat liver Cd,Zn-thionein. Some emphasis was placed on metal-binding and specificity properties. Cd-peptides of apparent Mr 6000 and 2000 were induced in tobacco leaves by growth of plants with 90 m...

The Maize Wip1 gene encodes a wound-induced Bowman-Birk inhibitor (BBI) protein which is a type of serine protease inhibitor, and its expression is induced by wounding or infection, conferring resistance against pathogens and pests. In this study, the maize Wip1 promoter was isolated and its function was analyzed. Different truncated Wip1 promoters were fused upstream of the GUS reporter gene a...

Topping stage of tobacco is a key time for development of agriculture measures to promote the quality of leaves. In order to investigating influence of topping timing and suckericide content on tobacco leaf, a factorial experiment on the basis of RCB design with 3 replications was conducted in 2011 at the Tobacco Research Farm of Isfahan. Treatments were including topping time (T1: t...

Vitamin E is a generic term for a group of lipid-soluble antioxidant compounds, the tocopherols and tocotrienols. While tocotrienols are considered as important vitamin E components in humans, with functions in health and disease, the protective functions of tocotrienols have never been investigated in plants, contrary to tocopherols. We took advantage of the strong accumulation of tocotrienols...

Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants.  Objective: The main goal of the present study was to eva...

The control of the curing temperature during stem-drying stage is important for quality upper leaves flue-cured tobacco. Based on conventional process yellowing and leaf0drying in tobacco process, this study, dry-bulb was increased to 72 75 °C bulk barn compare with highest 68 commonly adopted barn. result showed that, compared setting, energy consumption cost reduced by 0.10 or 0.08 USD/kg whe...

background: transient and stable transformation of host plants are the common techniques to produce transgenic plants. however, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. while, transient gene expression is a quick method to produce recombinant proteins in plants.  objective: the main goal of the present study was to eval...