A cellulase producing bacterium (E3 strain) was isolated from fecal matter of elephant and identified as Bacillus sp. using 16S rDNA sequenced based molecular phylogenetic approach. While studying the effect of substrates like Carboxymethyl cellulose (CMC), avicel, starch, maltose, sucrose, glucose, fructose, galactose and lactose on cellulase production, it was found that CMC was best carbon s...

In this study, the effect of xylanase and cellulase supplementation in palm kernel meal (PKM) based diet on growth performance, volatile fatty acids (VFAs) and the caecal bacterial populations of broiler chickens were investigated. Seventy five day old male Cobb broiler chicks were randomly allocated to three dietary treatment groups receiving T1 (20% PKM-based diet without enzyme), T2 (20% PKM...

Cellulase production by the fungus Trichoderma reesei was studied using sugar beet pulp (SBP) as a substrate. The subculture medium was a salt solution consisting of KH2PO4, CaCl2, etc. Fungal cells were sub-cultured in an orbital shaker (180 rpm) at 30°C for 1-2 generations (two days for each generation) and were then used as an inoculum. Exponential cells were inoculated into a medium contain...

Cellulose digestion in termites (Isoptera) is highly important for ecological reasons and applications in biofuel conversion. The speciose Termitidae family has lost flagellates in the hindgut and developed diverse feeding habits. To address the response of cellulase activity to the differentiation of feeding habits, a comparative study of the activity and distribution of composite cellulases, ...

The production of second generation biofuels -cellulosic ethanol from renewable lignocellulosic biomass has the potential to lead the bioindustrial revolution necessary to the transition from a fossil fuel-based economy to a sustainable carbohydrate economy. Effective release of fermentable sugars through biomass pretreatment followed by enzymatic hydrolysis is among the most costly steps for e...

Regulation of cell-specific cellulase synthesis (expressed in milligrams of cellulase per gram [dry weight] of cells) by Clostridium thermocellum was investigated using an enzyme-linked immunosorbent assay protocol based on antibody raised against a peptide sequence from the scaffoldin protein of the cellulosome (Zhang and Lynd, Anal. Chem. 75:219-227, 2003). The cellulase synthesis in Avicel-g...

leptinotarsa decemlineata (say) and lasioderma serricorne f. are destructive pre-harvest and post-harvest pests of many plants in the family solanaceae, and stored foodstuffs and non-food items, respectively. in this study, some biochemical characteristics of cellulase in the larval digestive tract of these pests were studied. endo-β-1, 4-glucanase activity was measured against the substrate ca...

Effective hydrolysis of pretreated lignocellulose mediated by cellulase requires an in-depth understanding of cellulase adsorption and desorption. Here we developed a simple method for determining the adsorbed cellulase on cellulosic materials or pretreated lignocellulose, which involves (i) hydrolysis of adsorbed cellulase in the presence of 10 M of NaOH at 121 degrees C for 20 min, and (ii) t...

Vatanparast M., Hosseininaveh V., Ghadamyari M., Minoo Sajjadian S. (2014): Plant cell wall degrading enzymes, pectinase and cellulase, in the digestive system of the red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae). Plant Protect. Sci., 50: 190–198. In digestion, the red palm weevil, Rhynchophorus ferrugineus, has been adapted to overcome the plant cell wall barrier, spec...