Total petroleum hydrocarbon (TPH) is a complex mixture of hydrocarbons including saturate fraction such as n-alkanes, branched alkanes, and cycloalkanes (naphthenes). TPH is normally come from the accidental leakage of storage tanks of lubricating and fuel oils resulting in the soil contamination. TPH is classified as the hazardous material and is considered as the most organic pollutants in th...

P.A. Kulakow and L. Erickson Department of Agronomy, Throckmorton Hall, Kansas State University, Manhattan, KS 66506; Phone: (785) 532-7239; e-mail: [email protected]. Department of Chemical Engineering, Durland Hall, Kansas State University, Manhattan, KS 66506; Phone: (785) 5324313; e-mail: [email protected]. ABSTRACT The TPH (total petroleum hydrocarbon) subgroup of the RTDF (Remediation Technolo...

We studied the effects of aged total petroleum hydrocarbons (aged TPH) on the survival of allochthonous diesel-degrading Rhodococcus sp. strain YS-7 in both laboratory and field investigations. The aged TPH extracted from a crude oil-contaminated site were fractionized by thin-layer chromatography/flame ionization detection (TLC/FID). The three fractions identified were saturated aliphatic (SA)...

This study evaluated the effects of aeration (constant aeration, intermittent aeration and a lack of aeration) and hydraulic retention time (HRT) (2, 3 and 4 days) on a continuous process with cell recycling, using chitosan as a natural coagulant for the sedimentation of a C1 mixed culture. This culture was used for the biodegradation of hydrocarbons present in the effluent contaminated with di...

Were evaluated natural attenuation, biostimulation and bioaugmentation on the degradation of total petroleum hydrocarbons (TPH) in soils contaminated with diesel oil. Bioaugmentation showed the greatest degradation in the light (C12 C23) fractions (72.7%) and heavy (C23 C40) fractions of TPH (75.2%) and natural attenuation was more effective than biostimulation. The greatest dehydrogenase activ...

Members of the Sphingomonas genus are often isolated from petroleum-contaminated soils due to their unique abilities to degrade polycyclic aromatic hydrocarbons (PAHs), which are important for in situ bioremediation. In this study, a combined phenotypic and genotypic approach using streptomycin-containing medium and Sphingomonas-specific PCR was developed to isolate and identify culturable Sphi...

Biodegradation of petroleum hydrocarbons in cold environments, including Alpine soils, is a result of indigenous cold-adapted microorganisms able to degrade these contaminants. In the present study, the prevalence of seven genotypes involved in the degradation of n-alkanes (Pseudomonas putida GPo1 alkB; Acinetobacter spp. alkM; Rhodococcus spp. alkB1, and Rhodococcus spp. alkB2), aromatic hydro...

Petroleum hydrocarbons are contaminants of particular significance. With production rates of 13.26 million cubic meters of crude oil per day, their potential as soil and water contaminant is apparent. The most commonly used analytic method for assessing Total Petroleum Hydrocarbons (TPH) in soil samples, is based on extraction with 1,1,2-Trichlorotrifluoroethane (Freon 113) , a substance prohib...

Abstract Various human activities have led to multiple contamination of natural water systems. The present study investigated the effect a novel multifunctional biochar treat nutrients, oil, and harmful algae in water. Specifically, magnesium (Mg) biosurfactant rhamnolipid (RL) were incorporated into biochar, including Mg-biochar, RL-biochar, Mg-RL-biochar. Their adsorption efficiency on phosph...