The glutathione S-transferases that were purified to homogeneity from liver cytosol have overlapping but distinct substrate specificities and different isoelectric points. This report explores the possibility of using preparative electrofocusing to compare the composition of the transferases in liver and kidney cytosol. Hepatic cytosol from adult male Sprague-Dawley rats was resolved by isoelec...

The mite Sarcoptes scabiei causes sarcoptic mange, or scabies, a disease that affects both animals and humans worldwide. Our interest in S. scabiei led us to further characterise a glutathione S-transferase. This multifunctional enzyme is a target for vaccine and drug development in several parasitic diseases. The S. scabiei glutathione S-transferase open reading frame reported here is 684 nucl...

Scoparia dulcis L. is widely used in the traditional system of medicine for treating liver ailments. In present study flavonoids and flavonoid fractions isolated from 9:1 Ethyl acetate methanol (EAM) extract were tested their vitro 1, 1- Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. Selected samples assay further hepatoprotective activity against CCl4 induced hepatotoxicity fres...

A hitherto unknown cytosolic glutathione S-transferase from rat liver was discovered and a method developed for its purification to apparent homogeneity. This enzyme had several properties that distinguished it from other glutathione S-transferases, and it was named glutathione S-transferase X. The purification procedure involved DEAE-cellulose chromatography, (NH4)2SO4 precipitation, affinity ...

The regulation of succinyl-CoA:acetoacetyI-CoA transferase (CoA transferase) has been studied in 8 rat hepatoma cell lines. Compared with normal rat hepatocytes, which have almost nondetectable activity of the enzyme, the hepatoma cell lines have a wide range of expression of CoA transferase activity, from as low as 45 nmol/min/mg to as high as 960 nmol/min/mg. \Yestern blotting showed that the...

The regulation of succinyl-CoA:acetoacetyl-CoA transferase (CoA transferase) has been studied in 8 rat hepatoma cell lines. Compared with normal rat hepatocytes, which have almost nondetectable activity of the enzyme, the hepatoma cell lines have a wide range of expression of CoA transferase activity, from as low as 45 nmol/min/mg to as high as 960 nmol/min/mg. Western blotting showed that the ...

Diphtheria toxin is known to catalyze a reversible reaction in which the adenosine diphosphate ribose moiety of NAD is transferred to aminoacyl transferase II, resulting in the concomitant loss of transferase activity in protein synthesis. Some general characteristics of the reaction are reported and a mechanism is proposed. The rate of the reaction is proportional to toxin, aminoacyl transfera...