Plants are an effective and inexpensive host for the production of commercially interesting heterologous recombinant proteins. The Escherichia coli-derived glutathione reductase was transiently expressed as a recombinant model protein in the cytosol of tobacco plants using the technique of leaf agro-infiltration. Proteolytic cysteine protease activity progressively increased over time when glut...

The adoption of pest-resistant transgenic plants to reduce yield loss and pesticide utilization has been successful in the past three decades. Recently, transgenic plant expressing double-stranded RNA (dsRNA) targeting pest genes emerges as a promising strategy for improving pest resistance in crops. The steroid hormone, 20-hydroxyecdysone (20E), predominately controls insect molting via its nu...

The wound-activated biosynthesis of phytoalexin hydroxycinnamic acid amides of tyramine was compared in untransformed and transgenic tobacco (Nicotiana tabacum) lines that express tryptophan decarboxylase (TDC), tyrosine decarboxylase (TYDC), or both activities. Transgenic in vitro-grown tobacco lines expressing TDC activity accumulated high levels of tryptamine but not hydroxycinnamic amides o...

expansin proteins are a type of cell-wall loosening proteins which able to cell wall expansion in ph-dependent manure without hydrolyze of main cell wall structures. the arabidopsis expa1 exhibit guard cell specific expression and trigger the stomatal movements. the objectives of this study were to transfer the atexpa1 gene into tobacco plants and evaluate their phonotypic effects on transforme...

NPR1 gene was transformed into Siraitia grosvenorii (Swingle) C. Jeffrey ex. A.M. Lu and Zhi Y. Zhang by germ cell in situ transformation. Ovary injection, cutting chapiter, and chapiter spreading treatments were applied in this study. The transgenic plants were selected using hygromycin screening and confirmed by PCR testing, genome integrated with NPR1 gene in transgenic plants was analyzed b...

The first intron of castor bean catalase gene, cat-1 was placed in the N-terminal region of the coding sequence of the beta-glucuronidase gene (gusA) and the intron-containing gusA was used with the cauliflower mosaic virus (CaMV) 35S promoter. Using this plasmid, pIG221, the effect of the intron on expression of beta-glucuronidase (GUS) activity was examined in transgenic rice calli and plants...