نتایج جستجو برای: transgenic tobacco plant
تعداد نتایج: 497382 فیلتر نتایج به سال:
Despite extensive studies in eukaryotic aldehyde dehydrogenases, functional information about the ALDH7 antiquitin-like proteins is lacking. A soybean antiquitin homologue gene, designated GmTP55, has been isolated which encodes a dehydrogenase motif-containing 55 kDa protein induced by dehydration and salt stress. GmTP55 is closely related to the stress-induced plant antiquitin-like proteins t...
In oilseed crops, a number of genes involved in the production of triacylglycerol have been identified that changes in their expression have increase the seed oil content. WRI1 and LPAAT are key genes in this synthetic pathway that their overexpression can increase the oil content. In this study, the expression vectors carrying WRI1 and LPAAT genes were designed and constructed for genetic tran...
expansin proteins are a type of cell-wall loosening proteins which able to cell wall expansion in ph-dependent manure without hydrolyze of main cell wall structures. the arabidopsis expa1 exhibit guard cell specific expression and trigger the stomatal movements. the objectives of this study were to transfer the atexpa1 gene into tobacco plants and evaluate their phonotypic effects on transforme...
Salinity as one of the most important stresses disturbs favorable growth conditions of the plants leading to several metabolic disorders such as reactive oxygen species (ROS). Monodehydroascorbate reductase (MDHAR) with FAD (Flavin adenine dinucleotide) co-factor is one of the key enzymes in Glutathione-ascorbate pathway which reduces monodehydroascorbate (MDHA) radicals. In this study MDHAR ge...
Background: Root hair culture is a valuable system to produce recombinant proteins in planta. Antimicrobial peptides (AMPs) are vital parts of the innate immune response found in almost all forms of life. Precise target activity and limited toxicity towards mammalian cells make them suitable candidate molecules to combat evolving drug-resistant microorganisms. The aim of the present study...
We present evidence that overproduction of endogenous cytokinins (CK) caused stress response in non-rooting Pssu-ipt transgenic tobacco (Nicotiana tabacum L.) grown in vitro. It was demonstrated by overaccumulation of phenolic compounds, synthesis of pathogenesis related proteins (PR proteins), and increase in peroxidase (POD) activities. Immunolocalization of zeatin and also PR-1b protein on l...
Phytosensors are useful for rapid-on-the-plant detection of contaminants and agents that cause plant stress. Previously, we produced a series of plant pathogen-inducible synthetic promoters fused to an orange fluorescent protein (OFP) reporter gene and transformed them into tobacco and Arabidopsis thaliana plants; in these transgenic lines, an OFP signal is expressed commensurate with the prese...
We have previously shown that the 15-amino acid carboxyl-terminal propeptide of probarley lectin is necessary for the proper sorting of this protein to the plant vacuole. A mutant form of the protein lacking the carboxyl-terminal propeptide is secreted. To test whether the carboxyl-terminal propeptide is the vacuole sorting determinant of probarley lectin, we examined in transgenic tobacco the ...
LSD1-related proteins of Arabidopsis with LSD1-like zinc finger domains regulate disease resistance and programmed cell death (PCD). We cloned a rice OsLOL2 gene, orthologous to LSD1 of Arabidopsis and expressed it in a tobacco plant. Transgenic tobacco lines displayed enhanced disease resistance to a virulent bacterium Pseudomonas syringae pv. tabaci (Pst). RT-PCR analysis showed that overexpr...
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