Amide peptide backbone methylation is a characteristic post-translational modification found in family of ribosomally synthesized and post-translationally modified natural products (RiPPs) called borosins. Previously, we bioinformatically identified >1500...

Protein arginine methylation is a widespread post-translational modification (PTM) in eukaryotic cells. This chemical proteins functionally modulates diverse cellular processes from signal transduction, gene expression, DNA damage repair,...

Many proteins, particularly those derived from eukaryotic sources, undergo covalent modification either during their ribosomal synthesis or (more usually) after synthesis is complete, giving rise to the concept of co-translational and post-translational modification. While the stage during protein synthesis/maturation at which the coor post-translational modification occurs obviously differs, b...

PII proteins are pivotal players in the control of nitrogen metabolism in bacteria and archaea, and are also found in the plastids of plants. PII proteins control the activities of a diverse range of enzymes, transcription factors and membrane transport proteins, and their regulatory effect is achieved by direct interaction with their target. Many, but by no means all, PII proteins are subject ...

In recent years, the number of recombinant proteins used for therapeutic applications and industry has increased dramatically. Recombinant proteins are produced in many host organisms (microbial, insect, plant and mammalian cells). There are many factors to consider when choosing the optimal system for protein expression and purification including the mass, purity or solubility of the recombina...

Post-translational protein modification, including phosphorylation, is generally quick and reversible, facilitating rapid biologic adjustments to altered cellular physiologic demands. In addition to protein phosphorylation, other post-translational modifications have been identified. Intracellular protein O-glycosylation, the addition of the simple sugar O-linked N-acetylglucosamine (O-GlcNAc) ...