Parolin, Michelle L., Alan Chesley, Mark P. Matsos, Lawrence L. Spriet, Norman L. Jones, and George J. F. Heigenhauser. Regulation of skeletal muscle glycogen phosphorylase and PDH during maximal intermittent exercise. Am. J. Physiol. 277 (Endocrinol. Metab. 40): E890–E900, 1999.—The time course for the activation of glycogen phosphorylase (Phos) and pyruvate dehydrogenase (PDH) and their allos...

Morris, R. Tyler, Espen E. Spangenburg, and Frank W. Booth. Responsiveness of cell signaling pathways during the failed 15-day regrowth of aged skeletal muscle. J Appl Physiol 96: 398–404, 2004. First published September 26, 2003; 10.1152/japplphysiol.00454.2003.— Various cellular signaling pathways, such as phosphatidylinositol 3-kinase, calcineurin, Janus kinase 2/signal transducer and activa...

Protein phosphorylation is one of the most common post-translational modifications regulating many cellular processes. The phos-tag technology was combined with two-dimensional zymograms, which consisted of non-reducing IEF PAGE or NEPHGE in the first dimension and high resolution clear native electrophoresis (hrCNE) in the second dimension. The combination of these electrophoresis methods was ...

CORREsPOnDEnCE TO: Janet Copeland Research and Professional development, new Zealand society of Physiotherapists, PO Box 27386, Wellington, new Zealand janet@physiotherapy. org.nz Although Primary Health Organisations (PHOs) were established to provide coordinated primary health care involving a range of providers, physiotherapy involvement in PHOs is limited. This means that access to physioth...

For over 150 years, hatchery-origin anadromous salmon and trout have been reared released throughout the Pacific Northwest to mitigate for lost habitat sustain harvest opportunity. Some studies demonstrate that introgression of hatchery naturally produced fish may constrain conservation efforts through maladaptive genetic processes. However, empirical demonstrations influence these interactions...

Protein phosphorylation is one of the most widespread regulatory mechanisms in eukaryotes. Over the past decade, phosphorylation site prediction has emerged as an important problem in the field of bioinformatics. Here, we report a new method, termed Random Forest-based Phosphosite predictor 2.0 (RF-Phos 2.0), to predict phosphorylation sites given only the primary amino acid sequence of a prote...

Immobilized metal ion affinity chromatography (IMAC) is now a widely accepted technique for the separation of natural or artificial products that is beginning to find industrial applications. Here, we introduce a novel procedure for the separation of phosphopeptides and phosphorylated proteins by immobilized zinc(II) affinity chromatography. The phosphate-binding site of the affinity gel is an ...

Lysophosphatidic acid (LPA) and sphingosine-1-phosphate (S1P) are growth factor-like lipids having a phosphate group. The concentrations of these mediator lipids in blood are considered to be potential biomarkers for early detection of cancer or vascular diseases. Here, we report a method for simultaneous determination of LPA and S1P using Phos-tag, a zinc complex that specifically binds to a p...