Desert truffles belonging to Terfezia are well known mycorrhizal members of the mycota of the Mediterranean region and the Middle East. We aimed to test (i) whether the morphological criteria of Terfezia species regularly collected in Spain enable their separation and (ii) whether the previously hypothesized edaphic/biotic specificity of one group could be confirmed by study of a larger number ...

Abstract In this study, we examined the effect of presence mycorrhiza and ascomata summer truffle ( Tuber aestivum ) on bacterial composition roots from small trees growing in selected sites Nida Basin. Qualitative DNA sequencing methods such as Sanger next-generation (NGS) were used. The method revealed different species compositions between samples where was recorded control samples. Five gen...

Background: The objective of this study was to analyze the current situation truffle sector in main producing countries Mediterranean area. Additionally, we identified challenges for future and priority actions develop region. Methods: We used a Delphi process approach, selected total 17 expert panelists different positions within supply chain target (Spain, France, Italy, Croatia, Greece). Res...

Black truffles that morphologically resemble Tuber indicum have been known to occur in Japan since 1979. Our previous studies showed that there are two phylotypes of these truffles, both of which fell into the T. indicum complex (hereinafter "Tuber sp. 6" and "Tuber sp. 7"). However, their taxonomic treatment is still unclear. In this study, we conducted morphological and phylogenetic analyses ...

Degenerate PCR primers were used to amplify a conserved gene portion coding chitin synthase from genomic DNA of six species of ectomycorrhizal truffles. DNA was extracted from both hypogeous fruitbodies and in vitro growing mycelium of Tuber borchii. A single fragment of about 600 bp was amplified for each species. The amplification products from Tuber magnatum, T. borchii and T. ferrugineum we...

BACKGROUND Tuber melanosporum, also known in the gastronomic community as "truffle", features one of the largest fungal genomes (125 Mb) with an exceptionally high transposable element (TE) and repetitive DNA content (>58%). The main purpose of DNA methylation in fungi is TE silencing. As obligate outcrossing organisms, truffles are bound to a sexual mode of propagation, which together with TEs...

The two morphologically similar truffles Tuber aestivum and T. uncinatum have caused confusion because T. uncinatum is regarded by different authors, as either a distinct species, variety, subspecies, or synonym of T. aestivum. A clarification of the relationship between the two truffles would help both conservation biology and cultivation. We aimed both to test the reliability of the only quan...

Arsenic and its species were investigated for the first time in nine collections of Elaphomyces spp. ("deer truffles") from the Czech Republic with inductively coupled plasma mass spectrometry (ICPMS) and high-performance liquid chromatography coupled to ICPMS. The total arsenic concentrations ranged from 12 to 42 mg kg-1 dry mass in samples of E. asperulus and from 120 to 660 mg kg-1 dry mass ...

Yield and different nut parameters were measured for two growing seasons on mature (28–29 years) trees of 11 pecan cultivars grown in an experimental orchard located the Puglia Region, Southeastern Italy. ‘Shoshoni’ ‘Shawnee’ seedlings inoculated with three truffle species (Tuber borchii—known as ‘whitish truffle’, T. aestivum—called ‘summer melanosporum—the common ‘Black truffle’) investigated...