When trypan blue is injected intravenously, the animal appears blue within a few minutes, not only in its integument, but in the internal organs as well. 12 or more hours after the administration of the dye, there are innumerable granules within macrophages and other colloidopexic cells, easily demonstrable by ordinary histological technique. But the animal is blue long before such granules are...

Background: Different cryoprotectants are used for Cryopreservation of ovarian tissue in patients who are at risk of infertility. Ethylene glycol (EG), dimethyl sulfoxide (DMSO) and propanediol (PROH) were chosen as the basic permeable cryoprotectants due to their less glass-formation characteristic compared to the others. In the present study, the effects of two different vitrification methods...

In this experiment, the action mechanism of gene Ssc1 was studied in process interaction with plants through heterologous expression, subcellular localization and fluorescent PCR technology. It found that could enhance pathogenicity Botrytis cinerea expression. Fusing promoter, SP CTP GFP expressing tobacco, it fusion protein be secreted into plant cells located chloroplasts. Trypan blue staini...

Erythrosin B and trypan blue are tested and compared for their effectiveness as vital exclusion stains for mammalian cells in monolayer culture. Both stains are supposed to mark cells that have lost membrane integrity. Fluorescein diacetate (FDA), an efficient vital inclusion stain, is used as a control, as it marks cells retaining membrane integrity. Erythrosin B and FDA are used as fluorescen...

OBJECTIVE Trypan blue and fluorescein sodium are two commonly used dyes in ophthalmic practice. The staining of anterior lens capsule is achieved using trypan blue while fluorescein finds its application in fluorescent angiography. We assessed the 'in vitro' bactericidal effect of these two agents. MATERIALS AND METHODS The cidal properties of these two dyes were tested in vitro against thirt...

We present a large comparative series of trypan blue use in cataract surgery. This series of trypan blue used in all eyes regardless of cataract severity may be unique. We found an apparent increased rate of cystoid macular oedema (CMO) associated with trypan blue use. Melles et al’s report on the use of trypan blue in cataract extraction in 1999 combined with Apple et al’s series on dye enhanc...

It has been reported that trypan blue treatment decreases the nonspecific resistance of mice to transplanted tumors and inhibits the in vitro cytotoxic activity of activated macrophages. We wished to determine whether this effect of trypan blue could be due to a selective inhibition of certain macrophage functions or whether it reflected a broader form of immunosuppression. We therefore tested ...