in present study, the kinetic parameters of purified trypsin from pyloric caeca of common kilka (c.cultriventris caspia) were measured using the amidolytic (bapna) and esterolytic (tame) substrates. the results of the kinetic comparison between bapna and tame substrates showed that kinetic parameters including maximum velocity (vmax), catalytic constant (kcat) and catalytic efficiency (kcat/km)...

Trypsin mRNA from the citrus weevil, Diaprepes abbreviatus, was reverse transcribed and amplified by PCR. A cDNA species of 513 bp was cloned and sequenced. The 3' and 5' ends of the gene (262 bp and 237 bp, respectively) were amplified by rapid amplification of cDNA ends, cloned and sequenced. The deduced sequence of the trypsin cDNA (860 bp) encodes for 250 amino acids including 11 amino acid...

Plasmodium falciparum invades erythrocytes through multiple ligand-receptor interactions, with redundancies in each pathway. One such alternate pathway is the trypsin-resistant pathway that enables P. falciparum to invade trypsin-treated erythrocytes. Previous studies have shown that this trypsin-resistant pathway is dependent on glycophorin B, as P. falciparum strains invade trypsin-digested g...

When crystalline trypsin was dissolved at various concentrations in pooled serum, the rates of hydrolysis of z-benzoyl-1-arginineamide (BAA) and N-benzoyI-dlarginine-/ -naphthylamide (BANA) were not linear functions of the trypsin concentration. Also, the plot of trypsin activity in relation to trypsin concentration differed with different sera. The apparent tryptic activity toward these substr...

colorectal cancer is one of the most common malignancy in the world and the second cancer-related death, many molecular and genetic aspects of this disease have been cleared as chromosomal instability and the role of some key proteins as wnt/? catenin, trypsin and others. also recently the role of folate turnover and some neurotransmitters as serotonin were also considered. the scope of this re...

Abstract Background Recent studies indicate that amylase-trypsin inhibitors (ATIs) and certain carbohydrates referred to as FODMAPs (fermentable oligo-, di-, monosaccharides polyols) play an important role in promoting wheat sensitivity. Hitherto, no study has investigated the accumulation of ATIs during development caryopsis. We collected caryopses common cv. ‘Arnold’ at eight different grain ...