Current methods for identifying transcription start sites (TSSs) of specific genes in bacteria usually require adaptors or radioactive labeling. These approaches can be technically demanding and environmentally unfriendly. Here we present a method for identifying TSS called ARF-TSS, which is based on cDNA generation, circularization, PCR amplification, and DNA sequencing to determine the 5'-end...

A quantitative comparative study was performed to assess the relative applicability of Total Suspended Solids (TSS) models published in the last decade for the Moderate Resolution Imaging Spectroradiometer (MODIS) and Landsat-based sensors. The quantitative comparison was performed using a suite of statistical tests and HydroLight simulated data for waters ranging from clear open ocean case-1 t...

BACKGROUND Malignant germ cell tumours represent the vast majority of palpable testicular masses, and radical orchiectomy is still considered the standard-of-care. Testis-sparing surgery (TSS) could be an alternative to radical orchiectomy in patients diagnosed with small testicular masses (STMs). The aim of this article was to review the current indications and the oncological and functional o...

OBJECTIVES To evaluate the reliability of a new scale, the Triple Spasticity Scale (TSS), for assessing spasticity in stroke, through measurement of affected elbow flexors and ankle plantar flexors of hemiplegic patients with stroke, and to compare the new scale with commonly used scales. DESIGN Cross-sectional study. SETTING Inpatients at a rehabilitation hospital. PATIENTS Seventy-one i...

Tetanic stimulation of the sciatic nerve (TSS) induces sciatic nerve injury and long-lasting pain hypersensitivity in rats, and spinal glial activation and proinflammatory cytokines releases are involved. In the present study, we showed that spinal interleukin (IL)-23 and its receptor, IL-23R, are crucial for the development of mechanical allodynia after TSS. In the spinal dorsal horn, both IL-...

This paper presents a comparison of U.S. Geological Survey stream monitoring station measurements of total suspended solids (TSS) yield and stream bank erosion monitoring data with a suspended solids model developed by Brown and Caldwell and used by the Gwinnett County, Georgia Department of Water Resources (DWR) to inform its watershed improvement efforts. A comparison between modeled and aver...

Spine is the primary bone site affected by systemic metastasis. Although there are scales that attempt to manage these patients, their real applicability is unknown. The Tokuhashi Scoring System (TSS) is a widely used prognostic tool. At the time of treatment, the data necessary to complete TSS may be incomplete, making its application impossible. Objective To evaluate the number of TSS scores ...

Bacteria respond to their environment by regulating mRNA synthesis, often by altering the genomic sites at which RNA polymerase initiates transcription. Here, we investigate genome-wide changes in transcription start site (TSS) usage by Clostridium phytofermentans, a model bacterium for fermentation of lignocellulosic biomass. We quantify expression of nearly 10,000 TSS at single base resolutio...

Based on an inverted bulk band order, antimony thin films presumably could become topological insulators if quantum confinement effect opens up a gap in the bulk bands. Coupling between topological surface states (TSS) from opposite surfaces, however, tends to degrade or even destroy their novel characters. Here the evolution and coupling of TSS on Sb(111) thin films from 30 bilayers down to 4 ...

The variety of access and transport technologies available in modern computer networks pose significant challenges related to compatibility and quality of service (QoS) related issues. Applications and services can have many different and unique requirements towards the transportation services (TSs) they use to interconnect. Traditionally, applications are required to specify their QoS requirem...