A potent tumor promoter, okadaic acid, induced hyperphosphorylation of tumor suppressor proteins, retinoblastoma protein and p53, by in vitro incubation with nuclei isolated from rat regenerating liver as well as by incubation with primary human fibroblasts. Most of the retinoblastoma protein migrated to a hyperphosphorylated position in electrophoresis. The phosphorylation of p53 was increased...

Keywords: tumor suppressor genes, TAGLN, cell lines, recombinant baculoviruses, relative gene expression

Molecular genetic studies of familial cancer syndromes identified and defined the recessive nature of tumor suppressor genes and resolved the paradox of why tumors arising in such families exhibited an autosomally dominant pattern of inheritance. Subsequent characterization of tumor suppressor proteins revealed their widespread involvement in sporadic cancers and pinpointed key mechanisms that ...

A potent tumor promoter, okadaic acid, induced hyperphosphorylation of tumor suppressor proteins, retinoblastoma protein and p53, by in vitro incubation with nuclei isolated from rat regenerating liver as well as by incubation with primary human fibroblasts. Most of the retinoblastoma protein migrated to a hyperphosphorylated position in electrophoresis. The phosphorylation of p53 was increased...

Mast cells (MCs) are important cellular components of the tumor microenvironment and significantly associated with poor patient outcomes in prostate cancer other solid cancers. The promotion progression partly involves heterotypic interactions between MCs cancer-associated fibroblasts (CAFs), which combine to potentiate a pro-tumor extracellular matrix promote epithelial cell invasion migration...

Cancer is one of the leading causes death worldwide. Multifactorial etiology cancer and tumor heterogeneity are two most acute challenges in existing diagnostic therapeutic strategies for cancer. An effective precision medicine strategy to overcome these requires a clear understanding transcriptomic landscape cells. Recent innovative breakthroughs high-throughput sequencing technologies have id...

Soluble proteins must protect their structural integrity from water attack by wrapping interactions which imply the clustering of nonpolar residues around the backbone hydrogen bonds. Thus, poorly wrapped hydrogen bonds constitute defects which have been identified as promoters of protein associations since they favor the removal of hydrating molecules. More specifically, a recent study of our ...

The tumor suppressor protein p53, a crucial player in the DNA damage response, is regulated in many ways, most notably through ubiquitination. In this issue, Yuan et al. (2010) identify the deubiquitinating protease USP10 as a new regulator of p53 in the DNA damage response and tumor development.