The current study was performed to clarify the effects of GHRH treatment on milk production and plasma hormones and metabolites in lactating Japanese Black cows (a beef breed) under negative energy balance (EB). Ten multiparous lactating beef cows were offered a normal-energy diet daily (110% of ME requirements for maintenance and lactation) until 5 d in milk (DIM) to standardize the cows befor...

A recent study found that an agarose gel electrophoresis (AGE) method yielded two distinct major bands corresponding to the hepatic and bone ALP isoenzymes (ALP2 ALP3, respec-tively) in bovine serum treated with protease neuraminidase (PN-treatment), although there were concerns intestinal isoenzyme (ALP5) often overlapped ALP3 human neuraminidase. Because ALP5 was separated from alone (P-treat...

A 70-day feeding trial was performed to assess the effect of a mixture functional feed supplements (FFS; contains encapsulated cinnamaldehyde, condensed tannins, capsaicin, piperine, and curcumin) during mid-lactation on milk production composition, intake, blood profile multiparous dairy cows. Sixty Holstein cows (116.1 ± 17.1 days in milk, 606 9.3 kg BW, 45.73 6.7 kg/d production) were distri...

this study was conducted to evaluate the effects of alfalfa hay (ah) particle size and soybean hull (sh) on physical effective fibre (pendf), particle size distribution in diets, chewing activity as well as milk composition and production in early lactating dairy cows. eight lactating holstein cows averaging 590±33 kg of bw and 47±13 days in milk were assigned in a 4×4 latin square design to a ...

Brangus cows (n = 29) were used in three experiments to evaluate the effects of parity (multiparous vs. primiparous) and potential genetic merit for milk production (high vs. low) on forage intake during late gestation, early lactation, and late lactation. Cows were selected for milk production based on their sire's EPD for milk production (MEPD). Cows had ad libitum access to (130% of previous...

We conducted three experiments to determine the influence of mechanical processing on corn silage utilization by lactating dairy cows. Total mixed rations contained either unprocessed or processed corn silage harvested between 1/4 and 3/4 milk line. In trial 1, 12 multiparous Holstein cows were used in a replicated double switchback design with 21-d periods. Intake of dry matter (DM) was increa...

Objectives were to evaluate effects of feeding a calcium salt rich in linoleic and trans-octadecenoic acids (LTFA) on synthesis of prostaglandin F(2alpha) based on its metabolite (PGFM), uterine involution and pregnancy rates in lactating dairy cows. Five hundred and eleven Holstein cows were blocked according to parity, body condition score and milk yield in the previous lactation. Primiparous...

This study aimed to investigate the effects of insulin resistance (IR) during the close-up dry period on the metabolic status and performance of dairy cows as well as to determine the effects on body weight (BW) and metabolic status of their calves. An insulin tolerance test (ITT) was conducted by administering 0.05 IU/kg BW of insulin to 34 multiparous Holstein cows at 3 weeks prepartum. Blood...

Heat stress has negative effects on pregnancy rates of lactating dairy cattle. There are genetic differences in tolerance to heat stress; Bos taurus indicus (B. t. indicus) cattle and embryos are more thermotolerant than Bos taurus taurus (B. t. taurus). In the present study, the effects of sire and sire breed on conception and embryonic/fetal loss rates of lactating Holstein cows during the Br...