Montero et al. do report the 95% limits on the Bland-Altman plot, perhaps the authors could have reported the 95% limits of agreement for a specific number of thresholds of proteinuria for example <300mg, <3.5 grams or >3.5 grams individually which would be useful for clini-cians trying to interpret spot urine pro-tein/creatinine ratio. I worry that readers of this excellent study may be left f...

antisense oligodeoxynucleotides (oligos) are the tools that bind to complemen¬tary sequence of targeted mrna and block specifically protein translation. in the present study, a novel 20 mer oligo as an antisense for human il-13 is introduced. this oligo is designed according to the il-13 mrna coding region and synthesized in two hplc purified and fitc conjugated forms. fluorescence oligo cell u...

While a main feature of HIV-1 pathogenesis is the death of CD4 ϩ T cells due to apoptosis, the mechanisms of apop-tosis are highly controversial (1, 2). Several HIV-1 proteins have been implicated in apoptosis regulation, among them viral protein R (Vpr), a small (‫ف‬ 14 kD) HIV-1 accessory protein that is packed in the nucleocapsid through its interaction with the Pr55 Gag precursor (3). In ad...

BACKGROUND Heart failure is operationally defined as the inability of the heart to maintain blood flow to meet the needs of the body and it is the final common pathway of various cardiac pathologies. Electrophysiological remodeling, intercellular uncoupling and a pro-fibrotic response have been identified as major arrhythmogenic factors in heart failure. OBJECTIVE In this study we investigate...

Among clinical conditions w ith increased tissue iron, id iopathic hem o­ chrom atosis is un ique. I t is associated w ith increased absorption and iron deposition in all tissues. E xperim ental m odels to study iron absorption and deposition in tissues m ay p rovide inform ation on the processes associated w ith excess iron uptake. Low pro tein high fat d iets induced excess iron deposition in...

Uncoupling protein 3L, uncoupling protein 1 and the mitochondrial oxoglutarate carrier were expressed in Saccharomyces cerevisae. Effects on different parameters related to the energy expenditure were studied. Both uncoupling protein 3L and uncoupling protein 1 reduced the growth rate by 49% and 32% and increased the whole yeast O2 consumption by 31% and 19%, respectively. In isolated mitochond...

1. To determine if there is an association between peroxisome proliferator activated receptor g (PPARg), agouti-related transcript (ART), neuropeptide Y (NPY) and its receptors NPY, Y5R and Y6R, pro-opiomelanocortin (POMC), uncoupling protein 2 (UCP2), melanocortin-4 receptor (MC4R) and obesity. 2. PCR of microsatellites regions within areas of suspected linkage will be used to identify candida...

Paper-strip electrophoretic studies using tris(hydroxymethyl)aminomethane-ethylene diaminetetraacetic acid-boric acid buffer were carried out on the serum of rats bearing primary methylcholanthrene-induced fibrosarcomas as well as 1st and 2nd transplant generation autografts and isografts of such tumors. It was found that, though bearing histologically similar tumors, serum of different rats pr...

background: allergy to saffron (crocus sativus) pollen has been described in people involved in processing of saffron flower stamens. profilins have been identified as a pan-allergen in different plant pollens and foods. this molecule is an actin-binding pro-tein with a molecular weight of 12-16 kda found in eukaryotic species. objective: the aim of this study was to generate monoclonal antibod...

Brown adipose tissue (BAT) cells have a very high oxidative capacity. On the other hand, in obesity and obesity-related diabetes, levels of pro-inflammatory cytokines are elevated, which might promote BAT dysfunction and consequently impair carbohydrate metabolism and thereby exacerbate cellular dysfunction and promote diabetes progression. Therefore, the antioxidative enzyme status of a brown ...