Objective(s): Tuberculosis (TB) has still remained a global health issue. One third of the world's population is infected with tuberculosis and the current BCG vaccine has low efficiency; hence, it is necessary to develop a new vaccine against TB. The aim of the current study was to evaluate the efficiency of a novel DNA vaccine encoding Mtb32C-HBHA antigen in inducing specific immune responses...

background and aims: in humans the group a rotaviruses are associated with endemic diarrhea in children under the age of 5, leading to approximately 800,000 deaths every year. introduction of rotavirus vaccines into childhood immunization programs can contribute to substantial reduction in mortality from rotavirus gastroenteritis in developing countries and virtually eliminating hospitalization...

avian pasteurella multocida is an agent of fowl cholera. the protective effect achieved through orthodoxvaccines is not ideal. the research on novel vaccines against avian pasteurella multocida is imperative. inthis study, the genes encoding outer membrane protein h and a (omph and ompa) were cloned into theeukaryotic expression vector pcdna3.1(+) and the recombinant plasmids, namely dna vaccin...

Objective(s): Infection with tuberculosis (TB) is regarded as a major health issue. Due to the emergence of antibiotic resistance during TB treatment, prevention via vaccination is one of the most effective ways of controlling the infection. DNA vaccines are developed at a greater pace due to their ability in generating a long-lasting immune response, higher safety com...

the identification of a large number of antigens with potential for development of new tuberculosis vaccine has been accomplished in recent years. this study was designed for cloning and expression of esat-6 as a potent antigen of mycobacterium tuberculosis. selected gene (rv3875) was amplified by pcr and product was ligated into expressing plasmid vector pqe30 and recombinant pqe30-es plasmid ...

Vaccination is one of the most successful immunology applications that has considerably improved human health. The DNA vaccine is a new vaccine being developed since the early 1990s. Although the DNA vaccine is promising, no human DNA vaccine has been approved to date. The main problem facing DNA vaccine efficacy is the lack of a DNA vaccine delivery system. Several studies explored this limita...

E1-deleted adenoviral recombinants most commonly based on the human serotype 5 (AdHu5) have been shown thus far to induce unsurpassed transgene product-specific CD8(+) T cell responses. A large percentage of the adult human population carries neutralizing Abs due to natural exposures to AdHu5 virus. To circumvent reduction of the efficacy of adenovirus (Ad) vector-based vaccines by neutralizing...

background and aims: dna constructs containing hcv antigens have become one of the vaccine candidates for induction of anti-hcv cellular and humoral immunity. in this study, we constructed a novel expressing vector harboring a fusion sequence derived from an overlapping fragment in the middle of ns3 and a truncated core fragment to avoid troubles reported to be associated with full gene express...