The effect of temperature on a very high gravity ethanol fermentation using no cook process was investigated. We found that a gradient temperature control strategy could improve the fermentation efficiency significantly, With the assistance of a new raw starch hydrolyzing enzyme and a gradient temperature control strategy, the ethanol concentration could reach up to 20% (V/V) within 90 h using ...

To intensify the brewing process and increase the fermenter productivity, many breweries throughout the world have been embracing high-gravity brewing. However, increasing original wort concentration can cause a negative effect on yeast fermentation performance. This study focused on the application of fed-batch fermentation in high-gravity brewing. High gravity worts of 24oBx (using 30% high m...

: In very high gravity brewing, fed-batch fermentation was carried out with Saccharomyces cerevisiae immobilized in calcium alginate gel. The specific gravity of initial wort and feeding medium originated from 55% malt and 45% maltose syrup adjunct was adjusted to 28oBrix and 41oBx, respectively. The effect of supplementation of mixture of Tween 80 and ergosterol to the initial wort or to the f...