ObjectiveUnplanned reintervention (uRE) is used as an indicator of patient morbidity and quality care in pediatric cardiac surgery. We investigated associated factors early mortality after uREs.MethodsMorbidity data were prospectively collected 5 UK centers between 2015 2017; uRE included surgical cardiac, interventional transcatheter permanent pacemaker, diaphragm plication procedures. Mortali...

User Requirements Elicitation (URE) is a critical stage in the development of software systems. Since it is communicative and interdisciplinary in nature, the process of URE implies a combination of soft skills and an active listening attitude, which can be trained only with the practice. In fact, despite a variety of techniques and approaches to URE are employed, and different methodologies ar...

In order to better understand pathogenicity of Helicobacter pylori, particularly in the context of its carcinogenic activity, we analysed expression of virulence genes: cagA, virB/D complex (virB4, virB7, virB8, virB9, virB10, virB11, virD4) and vacA in strains of the pathogen originating from persons with gastric diseases. The studies were conducted on 42 strains of H. pylori isolated from pat...

Quorum-sensing systems regulate the expression of virulence factors in a wide variety of plant and animal pathogens, including members of the Enterobacteriaceae. Studies of Shigella virulence gene expression have demonstrated that maximal expression of genes encoding the type III secretion system and its substrates and maximal activity of this virulence organelle occur at high cell density. In ...

The intracellular pathogenic bacterium Brucella generates a replicative vacuole (rBCV) derived from the endoplasmic reticulum via subversion of the host cell secretory pathway. rBCV biogenesis requires the expression of the Type IV secretion system (T4SS) VirB, which is thought to translocate effector proteins that modulate membrane trafficking along the endocytic and secretory pathways. To dat...

The α-proteobacterial genus Bartonella comprises a group of ubiquitous mammalian pathogens that are studied as a model for the evolution of bacterial pathogenesis. Vast abundance of two particular phylogenetic lineages of Bartonella had been linked to enhanced host adaptability enabled by lineage-specific acquisition of a VirB/D4 type IV secretion system (T4SS) and parallel evolution of complex...

In the study of state fai/ure, scholars and practitioners have mostly focused on macro-level indicators to determine which state isfailed and which is not. This focus overlooks cruciallocal and regional variation that has to be taken into account when designing interventions. In this article, we develop a new approach to examine state fai/ure on this microlevel. Using a unique data-set on Somal...

Urease-positive (Ure+) and urease-negative (Ure-) strains of Vibrio parahaemolyticus isolated from patients on the West Coast of the United States between 1979 and 1995 were analyzed for the thermostable direct hemolysin (tdh) gene and the tdh-related hemolysin (trh) genes (trh1 and trh2). The DNA colony hybridization method with the polynucleotide probes was used to determine the distribution ...

Canine brucellosis is a zoonotic disease caused by Brucella canis. The establishment of intracellular replicative niches of B. canis is mediated by proteins secreted by the type IV secretion system, which is encoded by the virB operon. The characterization of such genes has been conducted in other species of the genus, but not in B. canis. We report the design of a multiplex PCR test for the de...