Abstract With the development of bioinformatics, it is easy to obtain information and data about thousands genes, but determination functions these genes depends on methods for rapid effective functional identification. Virus-induced gene silencing (VIGS) a mature method identification developed over last 20 years, which has been widely used in many research fields involving species. Fruit qual...

Abstract Background Maize dwarf mosaic virus (MDMV), a member of the genus Potyvirus , infects maize and is non-persistently transmitted by aphids. Several plant viruses have been developed as tools for gene expression silencing in plants. The capacity MDMV both were examined. Results Infectious clones an Ohio isolate MDMV, OH5, obtained, engineered only, simultaneous marker virus-induced (VIGS...

Abstract Small RNAs (sRNAs) are a class of short, non-coding regulatory that have emerged as critical components defense networks across plant kingdoms. Many sRNA-based technologies, such host-induced gene silencing (HIGS), spray-induced (SIGS), virus-induced (VIGS), artificial microRNA (amiRNA) and synthetic trans -acting siRNA (syn-tasiRNA)-mediated RNA interference (RNAi), been developed dis...

RNA interference (RNAi) is a highly specific gene-silencing phenomenon triggered by dsRNA. This silencing mechanism uses two major classes of RNA regulators: microRNAs, which are produced from non-protein coding genes and short interfering RNAs (siRNAs). Plants use RNAi to control transposons and to exert tight control over developmental processes such as flower organ formation and leaf develop...

Ilex dabieshanensis is not only an important ornamental plant, but can also be used to produce Kuding tea, owing its lipid-lowering and anti-inflammatory medicinal properties. The genetic transformation of I. currently difficult, which restricts functional gene studies molecular breeding research on this species. Virus-induced silencing (VIGS) a powerful tool for determining functions in plants...

Virus-induced gene silencing (VIGS) is a powerful technique to study gene function in plants. However, very few VIGS vectors are available for monocot plants. Here we report that Foxtail mosaic virus (FoMV) can be engineered as an effective VIGS system to induce efficient silencing of endogenous genes in monocot plants including barley (Hordeum vulgare L.), wheat (Triticum aestivum) and foxtail...

Virus-induced gene silencing is currently a powerful tool for the study of gene function in plants. Here, we optimized the protocol for virus-induced gene silencing, and investigated factors that affect the efficiency of tobacco rattle virus-induced gene silencing in pepper plants. Consequently, an optimal protocol was obtained by the syringe-infiltration method in the leaves of pepper plants. ...

A recombinant Potato virus X (PVX) vector, pTXS.FtsH, harboring partial sequence of FtsH gene of Nicotiana benthamiana was constructed to silence the expression of endogenous FtsH homologous gene in N. benthamiana. Inoculation with in vitro runoff transcript of pTXS.FtsH to N. benthamiana plants allowed silencing of FtsH, causing striking bleaching of upper leaves reminiscent of var2 mutant phe...