When ecotropic murine leukemia virus was assayed by a methylcellulose-XC cell procedure, plaque titers showed less test-to-test variation, more uniform dose-response curves, and larger plaque sizes, as compared with results of the conventional liquid overlay-XC cell test system. This assay therefore seems to be reliable and useful for the titration of ecotropic murine leukemia virus.

INTRODUCTION Vaccines and antiviral drugs are the most widely used methods of preventing or treating Influenza virus infection. The role of sea buckthorn (SBT) bud dry extract as a natural antiviral drug against Influenza was investigated. METHODS Influenza virus was cultured in the MDCK cell line, with or without SBT bud extract, and virus growth was assessed by HA and TCID50 virus titration...

In this study, the pathogenicity of A/Ch/It/5093/1999 H7N1 which had been isolated from chicken during the outbreak in Italy was assessed in chicken by experimental infection virus. Ten SPF chickens of four week-old were inoculated with this virus, and five chickens were inoculated with uninfected allantoic fluid. For determination of virus shedding, oropharyngeal and cloacal swabs were taken f...

Background and Aims: Inhibition of viral growth in coinfected cells with two different viruses has been described. This phenomenon known as viral interference can occur in several virus host systems such as interference of enterovirus infection on poliovirus vaccine strains. In this study we superinfected reovirus infected HeLa cells with poliovirus to determine if poliovirus can replicate in s...

A characterization of the serological profile of 102 workers in constant activities in a veterinary hospital environment at the Universidade Federal de Mato Grosso was made through epidemiological data and serological tests to the rabies virus from November 1999 to November 2000. Of these, 27 had been vaccinated in a pre-exposure scheme and 75 had not received any kind of antirabies immunizatio...

bluetongue is an acute and subacute viral diseases of domestic and wild ruminants.this research was conducted to assess the disease status using serological diagnostic methods and virus isolation were performed. during the period 2003-2006, from sheep farms of 9 provinces including markazi, ardabil, yazd, qom, east azarbaijan, west azarbaijan, qazvin, isfahan and golestan, four handred thirty t...

The focus assay is currently the most commonly used technique for hantavirus titer determination. This method requires an incubation time of between 5 and 11 days to allow the appearance of foci after several rounds of viral infection. The following work presents a rapid Andes virus (ANDV) titration assay, based on viral nucleocapsid protein (N) detection in infected cells by flow cytometry. To...

Different naturally occurring, cell adapted or genetically manipulated stocks of African swine fever virus were able to infect directly cultures of COS-1 cells, producing extensive cytopathic effects and amounts from 10(6) to 10(7) of infective progeny virus per ml. The induction of late virus-specific proteins, demonstrated by RT-PCR and immunoblotting, and the development of lysis plaques by ...