We report a new method for automated affinity capture and release of biotin-containing conjugates on immobilized streptavidin using a lab-on-valve (LOV) bead injection apparatus. The apparatus is also coupled to UV/visible and electrospray ionization mass spectrometry (ESI-MS) for monitoring the captured and released biotin-containing conjugates. Dissociation rate constants for release from str...

Relative to other metabolomics analysis techniques, gas chromatography mass spectrometry (GC/MS) is one of the earliest applied analysis techniques in metabolomics. The first paper on metabolomics (metabolic profiling) is derived from the application of GC/MS analysis in urine and tissue extracts (Dalgliesh et al. 1966). With the arrival of omics era and the proposing of metabolomics concept, p...

A new ligand bearing two tpy moieties and one bpy unit (tpy = 2,2':6',2''-terpyridine; bpy = 2,2'-bipyridine) linked by carbon-carbon single bonds and its corresponding trinuclear ruthenium complex were readily synthesized in high yield, and characterized by (1)H NMR spectroscopy, high-resolution electrospray ionization mass spectrometry (HR-ESI/MS) and elemental analysis. The ruthenium complex...

Infared imaging process bears many similarities to the visible imaging process. If visible band computer vision techniques can be used on infrared images with no or small adjustments it would save us the trouble of redeveloping a whole new set of techniques. However, there are important differences in the practical environmental parameters between visible and infrared bands that invalidates man...