Our aim was to isolate and characterize white spot syndrome virus (WSSV)-binding proteins from shrimp. After a blot of shrimp hemocyte membrane proteins was overlaid with a recombinant WSSV envelope protein (rVP28), the reactive bands on the blot were detected using anti-VP28 antibody. Among three membrane-associated molecules identified by liquid chromatography-tandem mass spectrometry, there ...

White Spot Disease (WSD) is a viral disease affecting crustaceans. Caused by the Syndrome Virus (WSSV), this has caused significant mortality in commercially cultivated marine shrimp species with severe impacts on farming industry and may be threat to wild stocks. Thorough studies molecular biology of pathogen are urgently needed improve understanding virus at level, including variation key pro...

Despite the great commercial success, Pacific white shrimp Litopenaeus vannamei farming has suffered economic losses due to impact of viral diseases such as White Spot Syndrome. Among innovative approaches mitigate diseases, one most promising is manipulation eukaryote process known interfering RNA (RNAi). This natural mechanism can be induced improve degradation transcripts, through double-str...

Apoptosis plays an important role in white spot syndrome virus (WSSV) pathogenesis, and caspases are central players in apoptosis. Here, we cloned four novel caspases (Lvcaspase2-5) from the Pacific white shrimp Litopenaeus vannamei, and investigated their potential roles in WSSV replication using dsRNA-mediated gene silencing. Lvcaspase2-5 have the typical domain structure of caspase family pr...

Members of the microRNA miR-10 family are highly conserved and play many important roles in diverse biological mechanisms, including immune-related responses and cancer-related processes in certain types of cancer. In this study, we found the most highly upregulated shrimp microRNA from Penaeus vannamei during white spot syndrome virus (WSSV) infection was miR-10a. After confirming the expressi...