The coat protein of belladonna mottle virus (a tymovirus) was cleaved by trypsin and chymotrypsin, and the peptides were separated by high performance liquid chromatography using a combination of gel permeation, reverse phase, and ion pair chromatography. The peptides were sequenced manually using the 4-N, N-dimethylaminoazobenzene-4'-isothiocyanate/phenyl isothiocyanate double-coupling method....

abstract during the winter and spring of 2007 and 2008, 450 samples (leaves of carnation plants) showing symptoms of yellowing, mottling, leaf malformation, and stunting were collected from greenhouses in mashhad and chenaran regions. the samples were tested by das-elisa for the presence of carmv. total rna was extracted by rnxtm (-plus) solution from positive infected samples confirmed in das-...

We found that Sweet potato feathery mottle virus (SPFMV) P1, a close homologue of Sweet potato mild mottle virus P1, did not have any silencing suppressor activity. Remodeling the Argonaute (AGO) binding domain of SPFMV P1 by the introduction of two additional WG/GW motifs converted it to a silencing suppressor with AGO binding capacity. To our knowledge, this is the first instance of the trans...

watermelon mosaic virus is the most prevalent potyvirus infecting cucurbits in iran. in order to determine the distribution and frequency of the virus, 326 watermelon, melon, cucumber, winter and summer squash samples were collected from 22 villages around urmia during summer 2010 and tested by das-elisa with a polyclonal antibody. ninety four samples were detected as infected by elisa. to dete...