background & aim: the use of herbal extracts as safe and effective factors for eradicating of stores pests has attracted much attention. in this paper, the bio-effects of fumigation and repellent effects of essential oil of eucalyptus globolus were investigated on red flour beetle. experimental: in this study, the toxicity, fumigation and repellency contacts were assayed by topical applicat...

A novel DNA purification technology is described that enables the purification of pure dsDNA from blood. When compared to existing DNA purification methods, the Whatman BioScience Purification System (WBPS) offers a fast and convenient way to recover high yields of DNA. WBPS is based on a unique filter system that entraps DNA within a matrix. This allows the process to be performed in a single ...

The ability to use dry blood spots (DBSs) on filter paper for the analysis of urea levels could be an important diagnostic tool for areas that have limited access to laboratory facilities. We developed a method for the extraction and quantification of urea from DBSs that were stored on 3M Whatman filter paper and investigated the effect of long-term storage on the level of urea in DBSs. DBSs of...

BSA solutions of 10 %, 1 % and 0.1 % were prepared by addition of lyophilized BSA powder (Sigma Aldrich, St Louis, MO, USA) into deionized water before being filtered through a glass fiber filter (Whatman GF/F) under vacuum in order to remove particles that can clog the system. Tween 80 was then added to a obtain 0.05 % [V/V] solution. The BSA solutions were then refrigerated before use. All so...

BACKGROUND AND OBJECTIVES Glycated hemoglobin (HbA1c) provides an accurate and reliable method to assess the glycemic control in patients with Diabetes. Its measurement is limited by the inconvenience of sample collection that requires venipuncture, sample handling and storage factors. The aim of this study was to assess the feasibility of using a dried capillary blood spot on a filter paper to...

Point of care testing (POCT) as part diseases and health monitoring will be very beneficial in resource constrained areas with minimum healthcare expert power requirement. Elimination external source device brought the idea self-powered infusion micropump. This research aimed to fabricate investigate pumping capability non-mechanical micropump under effect different porous materials. The self-p...

The test is performed in closed vials at 100 oC for 5 days with the tested material and a reference paper in the vial. Volatiles off-gassed from the tested material may cause degradation of the reference paper and lead to a decrease in its degree of polymerization. This is determined using viscometry. The optimal vial type is proposed, leading to reproducible results with a standard deviation f...

We investigated whether dried cerebrospinal fluid (CSF) conserved on filter paper can be used as a substrate for accurate PCR diagnosis of important causes of bacterial meningitis in the Lao PDR. Using mock CSF, we investigated and optimized filter paper varieties, paper punch sizes, elution volumes and quantities of DNA template to achieve sensitive and reliable detection of bacterial DNA from...