نتایج جستجو برای: yeast expression vector

تعداد نتایج: 1122471  

Journal: :iranian journal of public health 0
hadi mirahmadi 1. dept. of parasitology and mycology, shahid beheshti university of medical sciences , tehran, iran . ; 2. dept. of parasitology and mycology, zahedan university of medical sciences , zahedan, iran. shirzad fallahi 3. dept. of parasitology and mycology, lorestan university of medical sciences , khoramabad, iran. vahid fallah omrani 1. dept. of parasitology and mycology, shahid beheshti university of medical sciences , tehran, iran . bahram kazemi 1. dept. of parasitology and mycology, shahid beheshti university of medical sciences , tehran, iran . ali haghighi 1. dept. of parasitology and mycology, shahid beheshti university of medical sciences , tehran, iran . seyyed javad seyyed tabaei 1. dept. of parasitology and mycology, shahid beheshti university of medical sciences , tehran, iran .

detection of plasmodium vivax specific antibodies with serological tests could be a valuable tool for epidemiological researches. whereas p. vivax cannot be simply obtained in vitro, serological tests using total or semi-purified antigens are infrequently used. given this restriction, the present study investigated whether recombinant p. vivax merozoite surface protein 1 (pvmsp-1 42 kda) could ...

Journal: :Microbial Cell Factories 2006
Gerhard Steinborn Erik Böer Anja Scholz Kristina Tag Gotthard Kunze Gerd Gellissen

BACKGROUND Yeasts provide attractive expression platforms in combining ease of genetic manipulation and fermentation of a microbial organism with the capability to secrete and to modify proteins according to a general eukaryotic scheme. However, early restriction to a single yeast platform can result in costly and time-consuming failures. It is therefore advisable to assess several selected sys...

2009
Thomas C Scanlon Elizabeth C Gray Karl E Griswold

BACKGROUND In addition to providing the molecular machinery for transcription and translation, recombinant microbial expression hosts maintain the critical genotype-phenotype link that is essential for high throughput screening and recovery of proteins encoded by plasmid libraries. It is known that Escherichia coli cells can be simultaneously transformed with multiple unique plasmids and thusly...

Ferulago angulata (Schlecht) Boiss is one of the valuable and endemic medicinal plants of Iran, which is of great importance due to the source of terpenoid compounds and antimicrobial properties. In current study, the effects of different concentrations of salicylic acid and yeast extract in cell suspension culture of F.angulata on expression pattern of the HMGR and GPPS genes (involved in terp...

Journal: :Nucleic acids research 1985
G T Marczynski J A Jaehning

YCp19 is a yeast centromere plasmid capable of autonomous replication in both yeast and E. coli (J. Mol. Biol., 158: 157-179, 1982). It is stably maintained as a single copy in the yeast cell and is therefore a model yeast "minichromosome" and cloning vector. We have located the positions and measured the abundance of the in vivo yeast transcripts from YCp19. Transcripts from the selectable mar...

Journal: :avicenna journal of medical biotechnology 0

background: recombinant monoclonal antibodies have been marketed in last three decades as the major therapeutic proteins against different cancers. however choosing a proper medium and supplements to reach the high expression is a challenging step. despite of commercial serum free and chemically defined media, there are still numerous researches seeking the optimum media to gain higher expressi...

Ali Zaree Mahmodabady, Ali Najafi, Hamid Reza Javadi, Mehdi Kamali, Zahra Hojati,

Background: RNA interference (RNAi) is the mechanism of gene silencing-mediated messenger RNA degradation by small interference RNA (siRNA), which becomes a powerful tool for in vivo research, especially in the areas of cancer. In this research, the potential use of an expression vector as a specific siRNA producing tool for silencing of Bcr-abl in K562 cell line has been investigated. Methods:...

Journal: :iranian journal of pathology 0
roghayeh teimourpour antimicrobial resistance research center, mashhad university of medical sciences, mashhad, iran amineh sadat tajani pharmacy school, mashhad university of medical sciences, mashhad, iran vahid reza askari pharmacy school, mashhad university of medical sciences, mashhad, iran sina rostami the influenza centre, department of clinical science, university of bergen, n-5021 bergen, norway zahra meshkat antimicrobial resistance research center, mashhad university of medical sciences, mashhad, iran

background: hepatitis c virus (hcv) infection is one of the most prevalent infectious diseases responsible for high morbidity and mortality worldwide. therefore, designing new and effective therapeutics is of great importance. the aim of the current study was to construct a dna vaccine containing structural proteins of hcv and evaluation of its expression in a eukaryotic system. methods: struct...

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