The extract of licorice (Glycyrrhiza glabra L.) has been widely used for many centuries in the traditional Chinese medicine as native anti-allergic agent. Glycyrrhizin (GL), a triterpenoid-saponin, extracted from the roots of licorice is the most effective compound for inflammation and allergic diseases in human body. The biological and pharmacological studies revealed that GL possesses many ph...

It is known that several antibiotics are inactivated by acetylation1"^. We have found that a blasticidin S (BS)producing actinomycete possesses an enzyme activity which acetylated BS in the presence of acetyl coenzyme A (acetyl CoA). The modified drug was biologically inactive6-1. The present study involves the isolation and biochemical properties of the enzyme. Streptoverticillium sp. JCM 4673...

It is well known that Glucagon (Gl) is released after a high protein diet and participates in water excretion by the kidney, principally after a protein meal. To study this effect in in vitro perfused inner medullary collecting ducts (IMCD), the osmotic water permeability (Pf; mum/s) at 37 degrees C and pH 7.4 in normal rat IMCDs (n = 36) perfused with Ringer/HCO(3) was determined. Gl (10(-7) M...

Alcoholic fermentation of cheese whey permeate was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus enabling for lactose metabolization. Data on yeast fermentation and growth on cheese whey permeate from a Portuguese dairy industry is presented. For...

The antibacterial activity of Artocarpus lakoocha Roxb. bark was tested against different species of bacteria. The minimum inhibitory concentration (MIC) of the methanolic extract showed that there was no growth in Shigella soneii 2, E.coli MTCC 1568, Bacillus pumilus 8241, Proteus mirabilis AM 198, Bacillus subtelis ATCC 6633, E. Coli Row 7/12 Species at 200-400g/ml. From the result obtained, ...

Yeast SRO7 was identified as a multicopy suppressor of a defect in Rho3p, a small GTPase that maintains cell polarity. Sro7p and Sro77p, a homologue of Sro7p, possess domains homologous to the protein that are encoded by the Drosophila tumor suppressor gene lethal (2) giant larvae [l(2)gl]. sro7Delta sro77Delta mutants showed a partial defect of organization of the polarized actin cytoskeleton ...

A homology-based PCR method was used to clone a cDNA encoding lanosterol synthase (LS) from Ganoderma lucidum (G. lucidum), which produces triterpenes. The cDNA of the LS (GenBank accession no. GQ169528) was found to contain an open reading frame (ORF) of 2,181 bp encoding a 726 amino acid polypeptide, whereas the LS genomic DNA sequence (GenBank accession no. GQ169529) consists of 2,924 bp. Fu...

The current epidemic of diabetes indicates the need of proper and effective medications that are limited in their potency to have many side effects. Thus, introduction of alternative and complementary medicine is now in picture. The main objective of this study was to analyze the rate of glucose transport across cell membrane in yeast cells system in the presence of the ethanolic extract of Bru...

background: recombinant monoclonal antibodies have been marketed in last three decades as the major therapeutic proteins against different cancers. however choosing a proper medium and supplements to reach the high expression is a challenging step. despite of commercial serum free and chemically defined media, there are still numerous researches seeking the optimum media to gain higher expressi...

the current epidemic of diabetes indicates the need of proper and effective medications that are limited in their potency to have many side effects. thus, introduction of alternative and complementary medicine is now in picture. the main objective of this study was to analyze the rate of glucose transport across cell membrane in yeast cells system in the presence of the ethanolic extract of bru...