Interleukin (IL) 12 is a 70-kD heterodimeric cytokine produced by antigen-presenting cells (APCs) such as macrophages in response to infectious pathogens and interferon (IFN) 3'. The varied immunomodulatory effects of I1-12 include the stimulation of proliferation and IFN-3, production by T cells, and it also has a central role in the development of the T hdper cell type 1 immune phenotype. We ...

Investigations of the interaction of cupric and zinc ions with sperm whale metmyoglobin (1) suggest that these ions interact at neutral pH with imidazole side chains and induce a reversible molecular denaturation not unlike that caused by acid (2). The binding affinity of sperm whale metmyoglobin for Cu(I1) ions and the number of protons released per mole of bound metal ion, moreover, suggest t...

Heterotrimeric G protein (G ) subunits possess intrinsic GTPase activity that leads to functional deactivation with a rate constant of 2 min 1 at 30°C. GTP hydrolysis causes conformational changes in three regions of G , including Switch I and Switch II. Mutation of G2023A in Switch II of G i1 accelerates the rates of both GTP hydrolysis and conformational change, which is measured by the loss ...

The continual detection of a slow (I1) esterase band in greenhouse cultures of Meloidogyne konaensis isolated from the field led to a hypothesis that the nematode may be polymorphic for esterase. A survey of coffee fields demonstrated at least four esterase phenotypes were present in Meloidogyne recovered. An F1 phenotype predominated (60% of the females), but an I1 phenotype was also common (3...

The ability of the aromatic diisocyanide ligand 1,4-diisocyanobenzene to act as a mediator for charge polarization between W(0) and W(I1) centers was investigated. A series of bridged complexes were synthesized which contained W(0)-W(O), W(0)-W(II), and W(I1)-W(I1) metal centers, coupled by CNC6H4NC. C-N stretching vibrations for the W(0) ends of the molecules were actually at higher energies t...

CLINICAL PHARMACOLOGY Absorption After oral administration, tinidazole is rapidly and completely absorbed. A bioavailability study of Tindamax tablets was conducted in adult healthy volunteers. All subjects received a single oral dose of 2 g (four 500 mg tablets) of tinidazole following an overnight fast. Oral administration of four 500 mg tablets of Tindamax under fasted conditions produced a ...

We study infinite-dimensional analogs of classical Lie superalgebras over an algebraically closed field F zero characteristic. Let I be infinite set. For algebra M_∞ (I) × matrices a ground having finitely many nonzero entries, we consider the related superalgebra gl_∞ (I1, I2) and its commutator sl_∞ for disjoint union nonempty subsets I1 I2 set I; describe derivations I2).

The metal ion dependence of a2P binding to Escherichia coli alkaline phosphatase has been studied by means of equilibrium dialysis. Whereas the apoenzyme does not bind phosphate (no sites with K < 5 X 10-5 M), the addition of 2 Zn(I1) cations per molecule induces the tight binding of 1 phosphate dianion (K = 6 X 1OF M). The magnitude of this binding constant is affected by ionic strength, pH, a...

Deprivation of cultured BRL-SA rat liver cells for a single essential amino acid (leucine, methionine, tryptophan, or phenylalanine) under conditions in which the cells remain highly viable causes a decreased secretion of insulin-like growth factor-I1 (IGF-11) E-domain peptide into the culture medium. This decrease is observed within 8 h after shifting the cells into amino acid-deficient medium...