نتایج جستجو برای: آنزیم ctx

تعداد نتایج: 15567  

2013
Ulzii-Orshikh Luvsansharav Itaru Hirai Marie Niki Arisa Nakata Aya Yoshinaga Akira Yamamoto Mayumi Yamamoto Hiroyuki Toyoshima Fusao Kawakami Nariaki Matsuura Yoshimasa Yamamoto

The detection rate of CTX-M-type β-lactamase-producing Enterobacteriaceae in Japan has significantly increased. Nursing homes may be a reservoir of antibiotic-resistant bacteria. Therefore, we determined the prevalence of, and risk factors associated with, fecal carriage of CTX-M-type β-lactamase-producing Enterobacteriaceae among nursing home residents. A total of 225 stool samples were collec...

ژورنال: :مجله دانشکده پزشکی اصفهان 0
مرسده تاجبخش کارشناس ارشد، دایره ی بیماری های ناشی از غذا و اسهال های مزمن، مرکز تحقیقات بیماری های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران محمد یعقوبی آوینی کارشناس ارشد، دایره ی بیماری های ناشی از غذا و اسهال های مزمن، مرکز تحقیقات بیماری های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران جهان علی خواجه استادیار، گروه بیوتکنولوژی، سازمان پژوهشهای علمی و صنعتی ایران، تهران، ایران مسعود آل بویه دکترای تخصصی باکتری شناسی، دایره ی بیماری های ناشی از غذا و اسهال های مزمن، مرکز تحقیقات بیماری های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران احسان ناظم الحسینی مجرد کارشناس ارشد، دایره ی بیماری های ناشی از غذا و اسهال های مزمن، مرکز تحقیقات بیماری های گوارش و کبد، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران محمدرضا زالی استادیار، گروه بیوتکنولوژی، سازمان پژوهشهای علمی و صنعتی ایران، تهران، ایران

مقدمه: یکی از مهم ترین مکانیسم های مقاومت به آنتی بیوتیک های گروه بتالاکتام تولید آنزیم بتالاکتاماز در باکتری ها است. هدف از مطالعه، بررسی نقش احتمالی میزان تنوع فعالیت آنزیم های بتالاکتاماز در بروز فنوتیپ های مقاومتی مختلف سویه های سالمونلای مولد بتالاکتاماز طیف گسترده (extended-spectrum beta-lactamases یا esbls) بود. روش ها: وجود فنوتیپ مقاومتی esbls در 174 جدایه ی سالمونلای بالینی پس از غربا...

Journal: :Antimicrobial agents and chemotherapy 2015
Dandan He Jiachi Chiou Zhenling Zeng Lanping Liu Xiaojie Chen Li Zeng Edward Wai Chi Chan Jian-Hua Liu Sheng Chen

A variety of CTX-M-type extended-spectrum β-lactamases (ESBLs), including hybrid ones, have been reported in China that are uncommon elsewhere. To better characterize the substrate profiles and enzymatic mechanisms of these enzymes, we performed comparative kinetic analyses of both parental and hybrid CTX-M enzymes, including CTX-M-15, -132, -123, -64, -14 and -55, that are known to confer vari...

Journal: :Antimicrobial agents and chemotherapy 2009
Yukiko Nagano Noriyuki Nagano Jun-ichi Wachino Keiko Ishikawa Yoshichika Arakawa

The plasmid-mediated novel beta-lactamase CTX-M-64 was first identified in Shigella sonnei strain UIH-1, which exhibited resistance to cefotaxime (MIC, 1,024 microg/ml) and ceftazidime (MIC, 32 microg/ml). The amino acid sequence of CTX-M-64 showed a chimeric structure of a CTX-M-15-like beta-lactamase (N- and C-terminal moieties) and a CTX-M-14-like beta-lactamase (central portion, amino acids...

Journal: :jundishapur journal of microbiology 0
hasan afzali department of infectious diseases, school of medicine, kashan university of medical sciences, kashan, ir iran farzaneh firoozeh department of microbiology and immunology, school of medicine, kashan university of medical sciences, kashan, ir iran; department of microbiology and immunology, school of medicine, kashan university of medical sciences, kashan, ir iran. tel/fax: +98-3155540021 atena amiri department of microbiology and immunology, school of medicine, kashan university of medical sciences, kashan, ir iran rezvan moniri department of microbiology and immunology, school of medicine, kashan university of medical sciences, kashan, ir iran mohammad zibaei department of parasitology and mycology, school of medicine, alborz university of medical sciences, karaj, ir iran

results of the 100 klebsiella isolates, 41 (41%) demonstrated resistance or reduced susceptibility to ceftazidime and/or aztreonam and 35% (n = 35) were esbl-producers. twenty-eight (8o%) of the esbl-producing isolates carried the blactx-m type genes. based on pcr assays and sequencing of blactx-m genes, ctx-m-1, ctx-m-2 and ctx-m-9 were identified in 21 (60%), 15 (42%) and nine (34%) of these ...

Journal: :Antimicrobial agents and chemotherapy 2013
Nathalie Grall Laurent Massias Thu Thuy Nguyen Sakina Sayah-Jeanne Nicolas Ducrot Elisabeth Chachaty Jean de Gunzburg Antoine Andremont

Antibiotics excreted into the intestinal tract, such as broad-spectrum cephalosporins, disrupt the indigenous microflora, affect colonization resistance (CR), and promote intestinal colonization by resistant bacteria. We tested whether oral DAV131, a charcoal-based adsorbent, would prevent colonization by a cefotaxime (CTX)-resistant Klebsiella pneumoniae strain (PUG-2) in CTX-treated mice. Mic...

Journal: :iranian journal of public health 0
m mirzaee dept. of microbiology, faculty of medicine, shahed university, tehran, iran mr pourmand dept. of pathobiology, school public health, tehran university of medical sciences, iran m chitsaz dept. of microbiology, faculty of medicine, shahed university, tehran, iran s mansouri dept. of microbiology, faculty of medicine, shahed university, tehran, iran

background: organisms producing ctx-m-β-lactamase are emerging around the world as a source of resistance to oxyiminocephalosporins such as cefotaxime. however, the laboratory detection of these strains is not well defined. the aim of this study was to determine the presence and prevalence of known ctx-m-β-lactamases genes in clinical isolates of  escherichia coli from hospitals of tehran. meth...

2011
Atupele Kapito-Tembo Steven R. Meshnick Michaël Boele van Hensbroek Kamija Phiri Margaret Fitzgerald Victor Mwapasa

BACKGROUND Effectiveness of cotrimoxazole (CTX) compared with sulfadoxine-pyrimethamine (SP) intermittent-preventive-therapy (IPTp) for malaria in HIV-infected pregnant women is unknown. We examined effectiveness of CTX with or without SP-IPTp versus SP-IPTp at reducing malaria parasitemia and anemia. METHODS From 2005 to 2009, we conducted a cross-sectional study of HIV-infected pregnant wom...

Journal: :Stroke 2007
Kon Chu Soon-Tae Lee Dong-In Sinn Song-Yi Ko Eun-Hee Kim Jeong-Min Kim Se-Jeong Kim Dong-Kyu Park Keun-Hwa Jung Eun-Cheol Song Sang Kun Lee Manho Kim Jae-Kyu Roh

BACKGROUND AND PURPOSE Astrocytic glutamate transporter protein, GLT-1 (EAAT2), recovers extracellular glutamate and ensures that neurons are protected from excess stimulation. Recently, beta-lactam antibiotics, like ceftriaxone (CTX), were reported to induce the upregulation of GLT-1. Here, we investigated ischemic tolerance induction by CTX in an experimental model of focal cerebral ischemia....

2013
Hisakazu Yano Mina Uemura Shiro Endo Hajime Kanamori Shinya Inomata Risako Kakuta Sadahiro Ichimura Miho Ogawa Masahiro Shimojima Noriomi Ishibashi Tetsuji Aoyagi Masumitsu Hatta Yoshiaki Gu Mitsuhiro Yamada Koichi Tokuda Hiroyuki Kunishima Miho Kitagawa Yoichi Hirakata Mitsuo Kaku

The prevalence of ESBL has been increasing worldwide. In this study, we investigated the molecular characteristics of ESBL among clinical isolates of Escherichia coli from a Japanese tertiary hospital. A total of 71 consecutive and nonduplicate clinical isolates of ESBL-positive E. coli collected at Tohoku University Hospital between January 2008 and March 2011 were studied. The antimicrobial s...

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