We report, the surface presentation of organophosphorus hydrolase (OPH) and green fluorescent protein (GFP) fusions by employing the adhesin-involved-in-diffuse-adherence (AIDA-I) translocator domain as a transporter and anchoring motif. The surface location of the OPH-GFP fusion protein was confirmed by immunofluorescence microscopy, and protease accessibility, followed by Western blotting ana...

As part of a maintenance cost reduction policy, the Royal Netherlands Navy RNLN recently funded the development, acquisition, certification and the fleetwide installation of a unique multi-channel on-board dataacquisition system for its Lynx helicopter, called AIDA (Automatic Inflight Data Acquisition). This 17-channel AIDA-system will generate valuable RNLN Lynx usage and loads data on main ro...

Many unsupervised anomaly detection algorithms rely on the concept of nearest neighbours to compute scores. Such are popular because there no assumptions about data, making them a robust choice for unstructured datasets. However, number (k) neighbours, which critically affects model performance, cannot be tuned in an setting. Hence, we propose new and parameter-free Analytic Isolation Distance-...

Myocardial infarction survivors have an increased risk of stroke, which is reduced with long-term anticoagulant therapy. However, an estimated 10-times increase in risk of bleeding during such treatment has been reported. We evaluated the risk of stroke in patients after a myocardial infarction and examined the relationship of the risk of intracranial hemorrhage or cerebral infarction and the i...

With the decreasing audience of classical music performance, this research aims to develop a performance-enhancement system, called AIDA, to help classical performers better communicating with their audiences. With three procedures Input-Processing-Output, AIDA system can sense and analyze the body information of performers and further reflect it onto the responsive skin. Thus abstract and inta...

This work addresses the research and development (R&D) of an innovative optimization kernel applied to analog integrated circuit (IC) design. Particularly, this work focus is AIDA-CMK, by enhancing AIDA-C with a new multi-objective multi-constraint optimization kernel. AIDA-C is the circuit optimizer component of AIDA, an electronic design automation framework fully developed in-house. The prop...

The proliferation of mobile computers and wireless networks requires the design of future distributed real-time applications to recognize and deal with the significant asymmetry between downstream and upstream communication capacities, and the significant disparity between server and client storage capacities. Recent work proposed the use of Broadcast Disks as a scalable mechanism to deal with ...

We present an NMR-based antagonist induced dissociation assay (AIDA) for validation of inhibitor action on protein-protein interactions. As opposed to many standard NMR methods, AIDA directly validates the inhibitor potency in an in vitro NMR competition binding experiment. AIDA requires a large protein fragment (larger than 30 kDa) to bind to a small reporter protein (less than 20 kDa). We sho...