نتایج جستجو برای: روش cbb
تعداد نتایج: 369922 فیلتر نتایج به سال:
Identifícation of RAPD markers linked to bacterial blight resistance genes in Phaseolous vulgaris L
Genetic studies indicate that resistance to bacterial blight is different depending on the source of resistance may be determined by both major and minor genes (Mcelroy 1985, Musaana el al, 1993. ). RAPD markers obtained from PCR amplification of genomic DNA with random primers have been successfully used for tagging genes of interest in common bean and other crops. For seed, pod and leaf react...
Can a heterotrophic organism be evolved to synthesize biomass from CO2 directly? So far, non-native carbon fixation in which biomass precursors are synthesized solely from CO2 has remained an elusive grand challenge. Here, we demonstrate how a combination of rational metabolic rewiring, recombinant expression, and laboratory evolution has led to the biosynthesis of sugars and other major biomas...
Methane is an important greenhouse gas and the most abundant hydrocarbon in the Earth's atmosphere. Methanotrophic microorganisms can use methane as their sole energy source and play a crucial role in the mitigation of methane emissions in the environment. "Candidatus Methylomirabilis oxyfera" is a recently described intra-aerobic methanotroph that is assumed to use nitric oxide to generate int...
DNA methylation is one of important epigenetics events, and responsible to transcription, genomic imprinting and cellular differentiation. Aberrant DNA methylation is always contacted with various diseases. Methyl binding domain (MBD) proteins can specifically bind to the methylated CpG dinucleotides. Conventional assay for DNA methylation normally need bisulfide treatment, methylated nucleotid...
In classical protein staining protocols using Coomassie Brilliant Blue (CBB), solutions with high contents of toxic and flammable organic solvents (Methanol, Ethanol or 2-Propanol) and acetic acid are used for fixation, staining and destaining of proteins in a gel after SDS-PAGE. To speed up the procedure, heating the staining solution in the microwave oven for a short time is frequently used. ...
Four manual micromethods for protein determination, two turbidimetric (trichloroacetic and sulfosalicylic acid-sodium sulfate) and two colorimetric (Lowry and Coomassie Brilliant Blue--sodium dodecyl sulfate, CBB-SDS) were used to compare the standard curves for total protein (0.30 to 3 g/L) produced with three reference materials: bovine serum albumin, human serum albumin, and diluted human se...
Software agents help automate a variety of tasks including those involved in buying and selling products over the Internet. This paper surveys several of these agentmediated electronic commerce systems by describing their roles in the context of a Consumer Buying Behavior (CBB) model. The CBB model we present augments traditional marketing models with concepts from Software Agents research to a...
Nitrosomonas europaea is an aerobic ammonia-oxidizing bacterium that participates in the C and N cycles. N. europaea utilizes CO(2) as its predominant carbon source, and is an obligate chemolithotroph, deriving all the reductant required for energy and biosynthesis from the oxidation of ammonia (NH(3)) to nitrite (). This bacterium fixes carbon via the Calvin-Benson-Bassham (CBB) cycle via a ty...
The coffee berry borer (CBB), Hypothenemus hampei, is the most destructive insect pest affecting plantations in coffee-producing countries, hence causing major economic losses worldwide. cryptic life cycle of CBB inside berries makes their control extremely difficult. To tackle this problem, we use a dynamical model describing plant–pest interactions during cropping season, which includes age s...
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