Comparative studies of aspartyl-, leucyl-, peripheral blood of a patient with miolseryland tyrosyl-tRNA by a reverse tiple myeloma and produce 1gM and phase column co-chromatography have lambda-type light chain (Bence Jones been performed between a human protein), respectively. Similar chromanormal lymphoblast cell line (RFMI tographic patterns were obtained for 1788) and a human myeloma cell l...

A spontaneously occurring antibasement membrane antibody has been further characterised according to antigenic specificity, immunoglobulin class, and tissue localisation using immunofluorescent and immunoelectron microscopic techniques. The autoantibody reacted with the basement membrane of kidney tubules, Bowman's capsule, and the epithelial portion of ileum but not with the basement membrane ...

Three diluents were studied, to determine which is the best for the automated immunochemical measurement of specific serum proteins. Nine serum proteins (orosomucoid, a 1-antitrypsin, a2-macroglobulin, haptoglobin, transferrin, C3, lgG, IgA, and 1gM) were measured in physiological saline (9 g NaCI/liter), tris(hydroxymethyl)aminomethane buffer (0.01 mol/liter; pH 7.4), and physiological saline ...

A radioirnmunoassay for SP1 (“pregnancy-specific” beta1-glycoprotein) is described in which a sensitivity of 0.1 tg/L was achieved by use of an antiserum with Ka 1.7 X lOll L/mol and delayed addition of a 1251-SP1tracer (specific acty, 25 Ci/g) that was stable for use in the assay for at least six to seven weeks. We also report an additional anti-SP serum, from an early bleeding, that displayed...

Although observations have implied that lupus anticoagulants have immunologic specificity toward anionic phospholipids. this assumption has been directly demonstrated in only one patient with a monoclonal 1gM paraprotein. We tested the generality of this hypothesis directly by isolating five lgG lupus anticoagulants from patients with lupuslike syndromes and/or thrombosis. lgG lupus anticoagula...

The ability of the tumor promotor 1 2-O-tetradecanoylphorbol 1 3-acetate (TPA) to induce differentiation of leukemic cells was studied in 1 0 cases of chronic lymphocytic leukemia (CLI). An increase in modal volume and an enhancement of the capacity of the leukemic cells to stimulate in mixed lymphocyte reaction (MLR) was seen in the majority of cases. A significant increase in Ia expression wa...

reductase inhibitors are the most widely used drug for the treatment of hyperlipidemias but their long term use is associated with rhabdomyolysis. Administration of simvastatin (80 mg/kg, p.o.) and gemfibrozil (600mg/kg, p.o. twice) for 30 days produced significant increase in the level of various serum parameters (creatine Phospho kinase, urea and blood urea nitrogen) indicating development of...

Serological and non-serological tests were performed in matched samples of cerebrospinalfluidand serum from236 syphilitic patients. An increased lgG or lgM index, or both, was found about70 times more often in symptomatic neurosyphilis than in latent syphilis without involvement of the central nervous system. An increased Ig index, together with a cell count >5/4., was only found in symptomatic...