In this study, we further investigated the mechanisms by which pseudophosphorylated prolactin (S179D PRL) inhibits the growth of human prostate cancer cells. When treated with S179D PRL for 3 days, LnCAP cells responded by increasing expression of the vitamin D receptor (VDR) and the cell cycle regulatory molecule, p21, whereas PC3 and DU145 cells did not. After 5 days of treatment, both PC3 an...

Background: Prostate cancer (Pca) is a heterogeneous disease, and current treatments are not based on molecular stratification. Poly(adenosine diphosphate [ADP]-ribose) polymerase (PARP) inhibitors have recently been found to be remarkably toxic to cells with defects in homologous recombination, particularly cells with BRCA-mutated backgrounds. Therefore, this preliminary study was designed to ...

Tumour necrosis factor α (TNFα) is involved in the pathogenesis of prostate cancer, a disease where disturbances in the endocannabinoid system are seen. In the present study we have investigated whether treatment of DU145 human prostate cancer cells affects anandamide (AEA) catabolic pathways. Additionally, we have investigated whether cyclooxygenase-2 (COX-2) can regulate the uptake of AEA int...

Prostate cancer (PCa) is a leading cause for death in men and the most commonly diagnosed malignancy globally. MicroRNA (miR)‑583 expression levels have been discovered to be downregulated recurrent PCa samples compared with non‑recurrent cases. However, precise functions pathogenic mechanism of miR‑583 development are vague, thus aim present study was investigate these. The Janus kinase 1 (JAK...

the purpose of this study is to investigate the effects of quercetin as an inhibitor of heat shock proteins and hyperthermia on the induced dna damages and colony formation ability of du145 tumor spheroid culture. du145 cells were cultured as spheroids. on day11, spheroids with mean diameter 100 μm were treated with different concentration of quercetin for 24 hours and then exposed to hyperther...

Prostate cancer is one of the most common malignancies in men, and it urgently demands precise interventions that target the signaling pathways implicated in its initiation, progression, and metastasis. The Notch-1 signaling pathway is closely associated with the pathophysiology of prostate cancer. This study investigated the antitumor effects and mechanisms of curcumin, which is a well-known n...

The epidermal growth factor receptor (EGFR) network has rich targets for prostate cancer killing. Herein we evaluated the effects of combining the EGFR inhibition and radiation on DU145 prostate cancer. We treated DU145 prostate cancer cells with various doses of anti-EGFR antibody (C225) and gamma-irradiation (RAD). The effects of the treatment on cell viability and growth were assessed with c...

Cancer metastasis is the process of dissemination cancer cells from their original site and localization in distant sites. During this journey, metastatic have to undergo several phenotypical/ genotypical changes for acquiring cell migration, invasion, attachment properties etc. These steps can be partially/fully mimicked under vivo vitro conditions. Here, we injected prostate line, DU145, into...