We have reported that heat shock protein 27 (HSP27) and annexin II are involved in the protection of human cells against UVC-induced cell death. In this study we tried to confirm the combined roles of HSP27 and annexin II in cell death after UVC irradiation. In RSa cells with sensitivity to UVC, expression of annexin II decreased after UVC irradiation, but not in AP(r)-1 cells with increased re...

Ultraviolet C (UVC) is a DNA damage inducer, and 20 J/m(2) of UVC irradiation caused cell growth inhibition and induced cell death after exposure for 24-36 h. The growth of NIH 3T3 cells was significantly suppressed at 24 h after UVC irradiation whereas the proliferation of A431 cells was inhibited until 36 h after UVC irradiation. UVC irradiation increased COX-2 expression and such up-regulati...

Emulation has been proposed as a preservation strategy for longevity of digital objects. An advantage of emulation is the relative platform independency. Emulation with a high degree of platform independency requires a Universal Virtual Computer (UVC), as described by Lorie. This paper describes the realization of the enhanced UVC developed for the National Library of the Netherlands. The prese...

In the event of a smallpox outbreak due to bioterrorism, delays in vaccination programs may lead to significant secondary transmission. In the early phases of such an outbreak, transmission of smallpox will take place especially in locations where infected persons may congregate, such as hospital emergency rooms. Air disinfection using upper-room 254 nm (UVC) light can lower the airborne concen...

PURPOSE To investigate whether low frequency pulsed magnetic field (PMF) exposures produce alterations in the cell killing induced by ultraviolet C (UVC) radiation. MATERIALS AND METHODS MCF-7 breast cancer cells of exponentially growing cultures were exposed to PMF (25 Hz, 0.75 mT) and UVC (from 6.6 J/m2 to 59.4 J/m2) in two different protocols: (a) cells were exposed to PMF for 30 min and t...

UV-based pathogen reduction technologies have been developed in recent years to inactivate pathogens and contaminating leukocytes in platelet transfusion products in order to prevent transfusion-transmitted infections and alloimmunization. UVC-based technology differs from UVA or UVB-based technologies in that it uses a specific wavelength at 254 nm without the addition of any photosensitizers....