Abstracfi This paper describes preparations of o(and r--) chlorolactic acids having enantiomeric excesses greater than 977o by o(and l-) lactate dehydrogenase catalyzed reduction of chloropyruvic acid with NADH. In syntheses carried out on 0.1{).5-mol scales, the NADH was regenerated in situ by using glucose 6-phosphate and glucose 6-phosphate dehydrogenase. The enantiomerically enriched chloro...

NADH oxidase-overproducing Lactococcus lactis strains were constructed by cloning the Streptococcus mutans nox-2 gene, which encodes the H2O-forming NADH oxidase, on the plasmid vector pNZ8020 under the control of the L. lactis nisA promoter. This engineered system allowed a nisin-controlled 150-fold overproduction of NADH oxidase at pH 7.0, resulting in decreased NADH/NAD ratios under aerobic ...

This paper describes experiments conducted with membranous and soluble fractions obtained from Escherichia coli that had been grown on succinate, malate, or enriched glucose media. Oxidase and dehydrogenase activities were studied with the following substrates : nicotinamide adenine dinucleotide, reduced form (NADH), nicotinamide adenine dinucleotide phosphate, reduced form (NADPH), succinate, ...

The involvement of nicotinamide adenine nucleotides (NAD(+), NADH) in the regulation of glycolysis in Lactococcus lactis was investigated by using (13)C and (31)P NMR to monitor in vivo the kinetics of the pools of NAD(+), NADH, ATP, inorganic phosphate (P(i)), glycolytic intermediates, and end products derived from a pulse of glucose. Nicotinic acid specifically labeled on carbon 5 was synthes...

NADPH nitrate reductase activity in higher plants has been attributed to the presence of NAD(P)H bispecific nitrate reductases and to the presence of phosphatases capable of hydrolyzing NADPH to NADH. To determine which of these conditions exist in barley (Hordeum vulgare L. cv. Steptoe), we characterized the NADH and NADPH nitrate reductase activities in crude and affinity-chromatography-purif...

The dicarboxylate radical -OOC--CH--CH(OH)COO- was generated in an N2O-saturated fumarate solution by high energy ionizing radiation. When NADH was present in the solution, product analysis indicated a stoichiometry of 2 molecules of the radical reacted with 1 NADH molecule to form 2 malate and 1 enzymatically active NAD+ molecules. In a similar experiment using tritium label on position A of N...

The NADH dehydrogenase of the Escherichiu coli respiratory chain has been identified by the following properties: (a) its location in membrane vesicles; (b) its inhibition by AMP in a fashion similar to that of the NADH oxidase; (c) its specificity for NADH, but not NADPH, with the same K, for NADH as that of the NADH oxidase; (d) its sensitivity when membrane-bound to inhibition by dicoumarol,...

Background/Aims: Cesium (Cs) is an alkali metal element that of no essential use for humans; it has known beneficial function verified by clinical research. When used as alternative cancer therapy, even causes toxicity in high doses. Thus, before using Cs treatment settings, important to clearly determine its biological effects on cells. However, was found suppress the proliferation human cervi...

Concentrative uptake of 16 amino acids by membrane vesicles isolated from Staphy2ococcus aureus is stimulated 3 to 100 times by the conversion of L-a-glycerol phosphate to dihydroxyacetone phosphate. With the exception of ascorbate-phenazine methosulfate, n-lactate, phosphoenolpyruvate, ATP, and a number of other metabolites and cofactors do not replace or-glycerol phosphate. Amino acid transpo...

Lactate dehydrogenase (LD: EC 1.1.1.27) is the most important clinically of several dehydrogenases occurring in human serum. Lactate dehydrogenase is cytoplasmic in its cellular location and in any one tissue is composed of one or two of five possible isoenzymes. While many of its clinical applications involve quantification of one or more specific serum isoenzymes, an estimate of total LD is r...