Lines. All Arabidopsis thaliana lines were in the Columbia background. The pin1 mutant allele used was pin1-7. DR5::GFP refers to the DR5rev::GFP line (1), in which the DR5 promoter consists of nine repeats of the DR5 element (2), fused in inverse orientation to the minimal CaMV 35S promoter and the coding sequence of an endoplasmic-reticulumtargeted eGFP protein. This construct was kindly prov...

Polymerase chain reaction (PCR) has been successfully employed to detect transgenes in genetically modified (GM) soybean and maize. Three pairs of primers for CaMV 35S, NOS and EPSPS genes were used in the detection. The results reveal that PCR could differentiate the GM plants from non-GM plants. Evidently, PCR is a highly reproducible and sensitive technique that can be successfully employed ...

potato (solanum tuberosum l.) an agro-economically important food crop in the world, is sensitive to many fungal pathogens including rhizoctonia solani (ag-3), the causal agent of stem and root rot diseases. chitinase and glucanase are cell wall degrading enzymes which have been shown to have high antifungal activity against a wide range of phytopathogenic fungi. in the present study, plasmid p...

Potato ( Solanum tuberosum L.) cultivar IPB CP1 is suitable as a raw material for the potato chip industry. plants are sensitive to various abiotic stresses such drought, aluminium and salinity, which induce reactive oxygen species (ROS). ROS very toxic plant cells. Superoxide dismutase (SOD) one of enzymes that catalyse H2O2 O2. This study aimed establish transgenic cv. containing MmCuZn‐SOD g...

Leaves of tomato cultivars that contain the Pto bacterial resistance locus develop small necrotic lesions within 24 hr after exposure to fenthion, an organophosphorous insecticide. Recently, the Pto gene was isolated and shown to be a putative serine/threonine protein kinase. Pto is one member of a multigene family that is clustered within a 400-kb region on chromosome 5. Here, we report that a...

Promoter regions associated with each of the six ssDNA components of banana bunchy top virus (BBTV) have been characterized. DNA segments incorporating the intergenic regions of BBTV DNA-1 to -6 were isolated and fused to the uidA (beta-glucuronidase) reporter gene to assess promoter activity. In tobacco cell suspensions, the BBTV DNA-2 and -6 promoters generated levels of GUS expression 2-fold...

Gene constructs containing the Cauliflower mosaic virus (CaMV) 35S promoter and a sequence coding either for a green fluorescent protein (GFP) or for firefly luciferase were transfected into Chinese hamster ovary (CHO) cells. Both reporter genes were expressed to significant levels. The 35S promoter was 40 times less active than the human eF1 alpha promoter, which is known to be one of the most...

We have characterized the upstream nucleotide sequences involved in mRNA 3'-end formation in the 3' regions of the cauliflower mosaic virus (CaMV) 19S/35S transcription unit and a pea gene encoding ribulose-1,5-bisphosphate carboxylase small subunit (rbcS). Sequences between 57 bases and 181 bases upstream from the CaMV polyadenylation site were required for efficient polyadenylation at this si...

In order to evaluate the functions of putative transcriptional regulatory regions of cotton genes, the upstream regulatory sequences (promoters) of two cotton genes, Gh-sp (seed protein) and Gh-rbcS (ribulose-1,5-bisphosphate carboxylase small subunit), were fused with a bb-glucuronidase (GUS) reporter gene and the cauliflower mosaic virus (CaMV) 35S terminator. These two gene constructs were t...