Dendritic cells (DCs) induce pathogen-specific T cell responses. We comprehensively studied the effects of addition of maturation stimulus, fibroblasts (fibroblast conditioned medium), PHA activated T cells (T cell conditioned medium), and mixture of fibroblast & PHA activated T cells (FCM-TCCM) conditioned media on maturation of DCs. Monocytes were cultured with GM-CSF and IL-4 for five days. ...

Acute myeloid leukemia (AML) is a type of poor prognosis hematological malignancies characterized by heterogeneous clonal expansion of myeloid progenitors. Leukemic stem cells are thought to form the majority of a cell population in minimal residual diseases (MRDs) which are resistant to current chemotherapeutic regimens and mediate disease relapse. Current therapeutic vaccine strategies have d...

the innate immune system utilizes pattern recognition receptors (prrs) to recognize microbes. pathogens contain various molecules with diverse effects on immune response. in this study, we evaluated the effect of dna and protein components derived from two intracellular microorganisms including listeria monocytogenes (l.monocytogenes) and toxoplasma gondii (t. gondii) on dendritic cells (dcs) a...