Guano samples from 412 Brazilian bats were screened with real-time PCR for the virulence genes (eae, est, elt, stx1, stx2, ehxA, invA, bfpA, aggR) representing five intestinal pathotypes of Escherichia coli. From 82 pooled samples, 22% contained Escherichia coli DNA, and eae, est, bfpA, aggR were detected.

Etiological diagnosis of diarrheal diseases may be complicated by their multi-factorial nature. In addition, Escherichia coli strains present in the gut can occasionally harbor virulence genes (VGs) without causing disease, which complicates the assessment of their clinical significance in particular. The aim of this study was to detect and quantify nine VGs (stx1, stx2, eae, aggR, ehxA, invA, ...

background: salmonella typhimurium is one of the most important species of salmonella that is intracellular parasite and attacks host mucus membrane. these bacteria can cause gastroenteritis, and their main transmission route is water, poultry, meat, egg, and raw food. the aim of this study was to detect three virulence genes associated with s. typhimurium named inva, stm4497, and flic183 genes...

This study was performed to investigate the prevalence of Salmonella spp. in 100 free freshwater common carp samples using the classic culture technique. For the confirmation of the isolates at molecular levels, the invA gene was detected. Serotyping of the isolates was also detected. The presence of invA, class 1 (Cls1) integrons, and integrase (Int1) genes was demonstrated by PCR assay; and th...

We find the Lie point symmetries for non–relativistic two-dimensional charged particle motion. These symmetries comprise a quasi–inva-riance transformation, a time–dependent rotation, a time–dependent spatial translation and a dilatation. The associated electromagnetic fields satisfy a system of first–order linear partial differential equations. This system is solved exactly, yielding four clas...

As part of a major international project for the validation and standardization of PCR for detection of five major food-borne pathogens, four primer sets specific for Salmonella species were evaluated in-house for their analytical accuracy (selectivity and detection limit) in identifying 43 Salmonella spp. and 47 non-Salmonella strains. The most selective primer set was found to be 139-141 (K. ...