Somatic mutations of the human mitochondrial genome can be a possible determinant of atherosclerosis. To test this possibility, forty mitochondrial mutations were analyzed in the present study in order to see which of these mutations might be associated with atherosclerosis. Ten mitochondrial mutations belonging to mitochondrial genes MT-RNR1 (rRNA 12S); MT-TL1 (tRNA-Leu, recognizes UUR); MT-TL...

Mice homozygous for a defect in the PTCD2 (pentatricopeptide repeat domain protein 2) gene were generated in order to study the role of this protein in mitochondrial RNA metabolism. These mice displayed specific but variable reduction of ubiquinol-cytochrome c reductase complex activity in mitochondria of heart, liver and skeletal muscle due to a decrease in the expression of mitochondrial DNA-...

The mitochondrial NADH dehydrogenase (complex I) in mammalian cells is a multimeric enzyme consisting of approximately 40 subunits, 7 of which are encoded in mitochondrial DNA (mtDNA). Very little is known about the function of these mtDNA-encoded subunits. In this paper, we describe the efficient isolation from a human cell line of mutants affected in any of these subunits. In the course of an...

Phylogenetic relationships among five species of Alosa (Alosa maeotica, A. tanaica, A. caspia, A. immaculata and A. fallax nilotica) was examined with sequence analyses of mtDNA ND5/6 region. In pairwise comparision, the highest genetic differences were observed between A. caspia and A. tanaica (0.691), and lowest between A. maeotica and A. tanaica (0.491). In minimum evolution tree, two phylog...

Rotary echoes are employed to study excitation dynamics in manybody Rydberg systems. In this method, a phase reversal of a narrow-band excitation field is applied at a variable time during the excitation pulse. The visibility of the resulting echo signal reveals the degree of coherence of the excitation process. Rotary echoes are measured for several nD5/2 Rydberg levels of rubidium with princi...

The hydrophobicity and hydrophilicity of amino acids play a very important role in protein folding and its interaction with the environment and other molecules, as well as its catalytic mechanism. Based on the two physicochemical indexes, a 2D graphical representation of protein sequences is introduced; meanwhile, a new numerical characteristic has been proposed to compute the distance of diffe...