background: stevia rebaudiana bertoni, an important anti-diabetic medicinal plant, becomes an inevitable alternative to sugar. due to the propagation difficulties, tissue culture is the best alternative for rapid mass propagation of stevia plants. objective: the present study was conducted to optimize a protocol for rapid micropropagation of stevia rebaudiana by shoot tip explants and to invest...

An efficient plant regeneration method was established for Caesalpinia bonduc (L.) Roxb. by culturingimmature epicotyl explants. Morphogenic calli were initiated from 96% of epicotyl explants on MS mediumsupplemented with BA (6-benzylaminopurine) 4.0 mg/l and NAA (Naphthalene acetic acid) 1.0 mg/l. The calliformed were excised and subcultured on MS medium fortified with 1 mg/l...

Generally, narcissus is propagated through vegetative methods, but due to their low yield their are not economical. This study was conducted to develop a suitable method for the production of somatic embryos and bulblets in Narcissus papyraceus cv. Shirazi. Bulb scales were placed on the MS medium supplemented with 2,4-D and BAP each at 0.0, 0.25, 0.5 or 1 mg/l concentrations to form somatic em...

who recommends artemisinin-based combination therapies (acts) as the most effective choice to treat malaria. for developing transgenic plants with high accumulation of artemisinin (by introducing genes encoding enzymes which regulate the biosynthetic pathway of artemisinin), an efficient protocol for tissue culture and plant regeneration is necessary. in the present study, leaf explants of arte...

punicagranatum cv. malassaveh is one of the most desirable commercial cultivars in iran. in thisstudy some experiments were conducted to introduce the best hormonal treatments for directadventitious shoot regeneration from mentioned cultivar via cotyledonary explants. for thispurpose, both liquid and agarmurashige and skoog (1962) (ms) medium supplemented with baat 2, 4.5, 9, 13 μm alone and in...

Sorghum is a wonder crop from physiological point of view. It is the most important cereal crop, after rice and wheat.  The number of reports describing the use of transgenic Sorghum for basic studies in Biotechnology is still limited when compared to other crops. In one hand, the success of the transformation techniques is mainly dependent upon the availability of optimal protocols for highly ...

to explore the potential for in vitro rapid regeneration of fagonia indica burm., cotyledon explants were obtained from 10-day old aseptically germinated seedlings that cultured on ms medium which supplemented with 1.0-5.0 µm 2,4-d. callus was initiated from cotyledon explant on ms medium supplemented in all concentrations of 2,4-d investigated. 2,4-d was more effective than iaa and naa for cal...