Introduction. Chronic persistent device-related infections (DRIs) often give culture-negative results in a microbiological investigation. In such cases, investigations on the device metagenome might have diagnostic value. Materials and Methods. The 16SrRNA gene sequence analysis next-generation sequencing (NGS) of clinical were performed to detect bacterial diversity invasive medical devices po...

Vibrio anguillarum is a pathogen that causes high mortality in marine and freshwater fish. The aim of this study was to develop a real-time PCR assay for identification and quantification of V. anguillarum in fish tissue. The assay was carried out using two target genes, 16SrDNA and toxR, to evaluate the influence of differences in the operon copy number in quantitative assessment, both in pure...

The level of cellulolytic activity in different areas of the gut of the terrestrial slug Arion ater was assayed at different temperatures and pH values. To do this, crude gut proteins were isolated and assayed using modified dinitrosalicylic acid reducing sugar assay. Crude protein samples were also separated and cellulolytic activity identified using in gel CMC zymography and esculin hydrate a...

Members of the bacterial population of two types of Portuguese dry smoked sausages (chouriços) were identified and characterized. A total of 77 isolates were recovered from chouriços type Alentejano and Ribatejano, after 120 days at 20 ± 5 oC in modified atmosphere package (55 % N2/45 % CO2) using non-selective and selective media. Based on phenotypic (cell morphology and fermentation profile) ...

We use fragments of three nuclear genes (Histone 3, 18SrDNA, and 28SrDNA) and three mitochondrial genes (16SrDNA, ND1, and COI) totalling approximately 4.5kb, in addition to morphological data, to estimate the phylogenetic relationships among Anelosimus spiders, well known for their sociality. The analysis includes 67 individuals representing 23 of the 53 currently recognized Anelosimus species...

objective: utility of pcr-rflp and species-specific pcr as novel and fast methods for identification and discrimination of causative agents of relapsing fever, borrelia persica and b. microtii in infected blood were investigated. materials and methods: genomic dna of b.persica and b.microtii species were extracted from the highly infected blood samples. two fragments of glpq and 16srdna genes ...

Tomato is one of the most important vegetables in the world. Decay after harvest is a major issue in the development of tomato industry. Currently, the most effective method for controlling decay after harvest is storage of tomato at low temperature combined with usage of chemical bactericide; however, long-term usage of chemical bactericide not only causes pathogen resistance but also is harmf...

BACKGROUND Pollution due to the heavy metals is a problem that may have negative consequences on the hydrosphere. One of the best procedures in removing the toxic metals from the environment is using metal resistant bacteria. RESULTS In the present study eight nickel resistant bacteria were isolated from industrial wastewaters. Three of them were selected as the most resistant based on their ...

BACKGROUND AND OBJECTIVES Ensiling is a conservation method for forage crops. It is based on the fact that anaerobe lactic acid bacteria (LAB) convert watersoluble carbohydrates into organic acids. Therefore, pH decreases and the forage is preserved. The aim of this study was to isolate special kinds of lactic acid bacteria from silage and to study the effect of bacteria, inulin and enzymes as ...

In this study, the ability of adsorbing Cd2+ ions of Plesiomonas shigelloides was discovered. Herein, the method and mechanisms of adsorbing Cd2+ ions from aqueous solutions is discussed. The cadmium-resistant bacterium was collected from the sediment of Harbin section of the Songhua River in China, and then isolated, identified and characterized. The isolated strain was identified as Plesiomon...