DRUGS AND UPTAKE OF 32p BY ERYTHROCYTES 533' 5. Sodium salicylate and sodium thiopentone depressed the 32p uptake of 2:3-diphosphoglycerate and adenosine triphosphate to a greater extent than they depressed the 32P exchange of inorganic phosphate and hexose monophosphate. These agents do not depress 32p uptake by inhibiting triose phosphate dehydrogenase, for neither drug had any effect on glyc...

The dominant insulin-stimulated ribosomal protein S6 kinase activity was purified to near homogeneity from insulin-treated 32P-labeled rat H4 hepatoma cells and found to copurify with a 70-kDa 32P-labeled polypeptide. The dominant S6 kinase purified from livers of cycloheximide-treated rats is also a 70-kDa polypeptide. Antiserum raised against rat liver S6 kinase specifically immunoprecipitate...

It was observed that rat liver cytosol can transfer 32P from 32PO4 to acceptor proteins. 32P-labeled proteins co-purify with a class of proteins known to accept arginine at their NH2-terminal end. When arginine-14C is added to the incubation mixture, 32P incorporation is stimulated and small molecules bearing both 32P and 14C labels can be released by treating labeled proteins with leucine amin...

The photoaffinity label 8-azido[32P]adenosine 3':5'-monophosphate (8-azido-cyclic [32P]AMP) was used to analyze both the cAMP-binding component of the purified cAMP-dependent protein kinase, and the cAMP-binding proteins present in crude tissue extracts of bovine cardiac muscle. 8-Azido-cyclic [32P]AMP reacted specifically and in stoichiometric amounts with the cAMP-binding proteins of bovine c...

The human asialoglycoprotein receptor was isolated via immune precipitation from hepatoma Hep G2 cells following incubation with [32P]Pi. Analysis on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis revealed incorporation of 32P into both the 46 000 Da mature form of the receptor as well as the 40 000 Da precursor. The incorporated 32P was associated with phosphoserine. The degree of ...

Infected cell protein 4 (ICP4), the product of the alpha 4 gene, regulates herpes simplex virus 1 and herpes simplex virus 2 gene expression at the transcriptional level both positively and negatively. Previous studies have shown that ICP4 is extensively modified posttranslationally. We report that ICP4 was labeled in isolated nuclei of infected cells by [alpha-32P]GTP or [alpha-32P]ATP. The la...

The purpose of this investigation was to examine the effects of beta-adrenergic and muscarinic cholinergic agonists on protein phosphorylation in intact frog atrium. beta-Adrenergic agonists increase and muscarinic agonists decrease 32P incorporation into a 165,000-dalton (165K) protein within less than 1 min. The concentrations of isoproterenol that produce increases in 32P incorporation into ...

Interactions between a murine monoclonal anti-DNA autoantibody (BV17-45) and DNA were examined by direct binding and competitive radioimmunoassays. Binding isotherms constructed by titration of purified BV17-45 with a series of distinct 32P-labeled double-stranded DNA ([32P]dsDNA) fragments were super-impossible, suggesting: 1) BV17-45/[32P]dsDNA binding is independent of dsDNA size using fragm...

Murine immune interferon (Mu-IFN-gamma) can be radiolabeled with [gamma-32P]ATP by the catalytic subunit of cAMP-dependent protein kinase. The resulting 32P-labeled Mu-IFN-gamma (32P-Mu-IFN-gamma) with high radiological specific activity (60-260 muCi/micrograms) retains biological activity. Acid hydrolysis of 32P-Mu-IFN-gamma or 32P-labeled human IFN-gamma leads to the release of [32P]phosphose...