The risk of chlorpyrifos (CPY) to aquatic organisms in surface water of North America was assessed using measured concentrations in surface waters and modeling of exposures to provide daily concentrations that better characterize peak exposures.Ecological effects were compared with results of standard laboratory toxicity tests with single species as well as microcosm and mesocosm studies compri...

We have studied components of the endoplasmic reticulum (ER) proofreading and degradation system in the yeast Saccharomyces cerevisiae. Using a der3-1 mutant defective in the degradation of a mutated lumenal protein, carboxypeptidase yscY (CPY*), a gene was cloned which encodes a 64-kDa protein of the ER membrane. Der3p was found to be identical with Hrd1p, a protein identified to be necessary ...

Respiratory syncytial virus (RSV) is a common cause of lower respiratory tract illness in infants, young children, and the elderly. The G glycoprotein plays a role in host cell attachment and also modulates the host immune response, thereby inducing disease pathogenesis. We generated two monoclonal antibodies (mAbs; 5H6 and 3A5) against G protein core fragment (Gcf), which consisted of amino ac...

The asymmetric unit of the title complex, [Zn3(C7H5O2)6(C6H4N2)2], contains one half of the complex mol-ecule, i.e. one and a half ZnII cations, three benzoate (Bnz) and one pyridine-3-carbo-nitrile (Cpy) mol-ecule; the Bnz anions act as bidentate ligands through the carboxyl-ate O atoms, while the Cpy ligand acts as a monodentate N(pyridine)-bonding ligand. The complete centrosymmetric trinucl...

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 208 No. 2 197–209 www.jcb.org/cgi/doi/10.1083/jcb.201408024 JCB 197 Correspondence to Charles Barlowe: [email protected] R. Christiano and T.C. Walther’s present address is Dept. of Genetics and Complex Diseases, Harvard School of Public Health, Boston, MA 02115. Abbreviation used in this paper: ALP, alkaline phosphatase; COPI, coat...

In the first paper in this series, a new integral equation approximation t h e Percus-Yevick excess (PYX) approximation--was introduced and contrasted with the corrected Percus-Yevick (CPY) approximation. In the present paper, these two approximations are evaluated for a molecular fluid, the molecules of which interact via a short ranged anisotropic potential prototypical of the overlap interac...

We are studying intercompartmental protein transport to the yeast lysosome-like vacuole with a reconstitution assay using permeabilized spheroplasts that measures, in an ATP and cytosol dependent reaction, vacuolar delivery and proteolytic maturation of the Golgi-modified precursor forms of vacuolar hydrolases like carboxypeptidase Y (CPY). To identify the potential donor compartment in this as...

OBJECTIVE To prepare monoclonal antibodies (MAb) and antisera specific for Escherichia coli (E. coli) O157, and to develop a sandwich enzyme-linked immunosorbent assay (ELISA) to detect E. coli O157 in foods. METHODS Spleen cells from BALB/c mice immunized with the somatic antigen of E. coli O157:H7 were fused with murine Sp2/0 myeloma cells. The hybridoma cell line specific for E. coli O157 ...

Toward a detailed understanding of protein sorting in the late secretory pathway, we have reconstituted intercompartmental transfer and proteolytic maturation of a yeast vacuolar protease, carboxypeptidase Y (CPY). This in vitro reconstitution uses permeabilized yeast spheroplasts that are first radiolabeled in vivo under conditions that kinetically trap ER and Golgi apparatus-modified precurso...

hen yeast secretory proteins are improperly folded, the ER quality control system directs them into the HRD/DER pathway, which targets the proteins for degradation. Blocking the HRD/DER pathway, however, does not completely stop degradation, implying that there must be an alternative way to destroy misfolded secretory proteins. Haynes et al., reporting on page 91, have now characterized that pa...