CLH-3a and CLH-3b are Caenorhabditis elegans ClC channel splice variants that exhibit striking differences in voltage, Cl(-), and H(+) sensitivity. The major primary structure differences between the channels include a 71 amino acid CLH-3a N-terminal extension and a 270 amino acid extension of the CLH-3b C-terminus. Deletion of the CLH-3a N-terminus or generation of a CLH-3a/b chimera has no ef...

Background: Knowing the human platelet angens (HPA) and genes frequency in different populaons is important not only for populaon studies but also for clinical transfusion pracce. HPA genes frequency in Iran is not evaluated, so we invesgated the HPA-2, HPA-3, and HPA-5 frequency and their polymorphism. Materials and Methods: DNA from 120 Iranians (99 Iranian blood donors and 21 paents wi...

The main objectives of this work were to characterise a range of purified recombinant sterol 3b-glucosyltransferases and show that rational sampling of the diversity that exists within sterol 3b-glucosyltransferase sequence space can result in a range of enzyme selectivities. In our study the catalytically active domain of the Saccharomyces cerevisiae 3b-glucosyltransferase was used to mine put...

Hen ovomucoid contains a series of novel pentaantennary complex-type asparagine-linked sugar chains which can be classified into three groups, N-1, N-2, and N-3b, N-3b, the structure of which is found as a common (formula, see text) structure in all pentaantennary sugar chains. N-2 is a mixture of three isomeric monogalactosyl derivatives of N-3b, and N-1 is a mixture of two isomeric digalactos...

Picornavirus RNA replication is initiated by a small viral protein primer, 3B (also known as VPg), that is covalently linked to the 5' terminus of the viral genome. In contrast to other picornaviruses that encode a single copy of 3B, foot-and-mouth disease virus (FMDV) encodes three copies of 3B. Viruses containing disrupted native sequence or deletion of one of their three 3B proteins were der...

Objective—Glycogen synthase kinase (GSK)-3b is a crucial factor in many cellular signaling pathways and may play an important role in smooth muscle proliferation and apoptosis after angioplasty. Methods and Results—To investigate the effect of GSK-3b modulation on neointima formation, smooth muscle proliferation, and apoptosis after balloon injury in vivo, we delivered adenoviral vectors expres...

Biacetyl reacts with oxone to give bis-dioxirane [3,3'-dimethyl-3,3'-bidioxirane, 3B] and mono-dioxirane [1-(3-methyl-dioxiran-3-yl)ethanone, 3A)]. Bis-dioxirane 3B is formed when two oxygens are incorporated into biacetyl, while mono-dioxirane 3A incorporated only one. A greater stability is observed in 3B compared to 3A, which is attributed to an alpha-dioxiranyl (anomeric) effect in the form...

BALB/c.DBA/2 Idh-1b-Ityr-Pep-3b congenic mice were developed by introgressively backcrossing the Idh-1b and Pep-3b markers of DBA/2 mice onto the BALB/c pi mice. This introduced a 30-centimorgan chromosome 1 segment of DBA/2 chromatin that contained the Ityr gene. BALB/c.DBA/2 Idh-1b-Ityr-Pep-3b mice were resistant to in vivo infections by Salmonella typhimurium, Mycobacterium bovis, and Leishm...