The senescence of excised discs of primary leaves of Phaseolus vulgaris, L., var. Red Kidney was followed by measuring the net breakdown of protein and chlorophyll. The chemical growth regulators indoleacetic acid, 2,4-dichlorophenoxy-acetic acid, gibberellic acid, kinetin, and 6-benzylaminopurine were relatively ineffective in retarding senescence in this tissue. White light, on the other hand...

The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult 'Hamlin', 'Pêra', and 'Valência' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27oC in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regen...

Saffron (Crocus sativus L.) protoplasts were isolated from the cells of a suspension culture or calli with a solution of Cellulase, Pectinase and Hemicellulase and embedded in Ca-alginate beads. They were cultured with or without nurse cells in MS medium supplemented with 2,4-D and 6-benzylaminopurine at 25°C. After several changes of medium, cell-clusters appeared on the surface of the Ca-algi...

Upon addition of 1-naphthaleneacetic acid (1-NAA) and benzylaminopurine, flower buds developed on explants from flower stalks of Nicotiana tabacum L. cv Samsun cultured in vitro. At low concentrations of 1-NAA, buds emerged mainly at the basal edge, whereas at high concentrations they developed on the remaining surface. The optimum concentrations for the two groups of buds were 0.45 micromolar ...

An effi cient and reproducible plant regeneration procedure is essential for introducing genes of interest in important crops through genetic transformation. However, some crops, such as soybean [Glycine max (L.) Merrill], are diffi cult to manipulate in vitro, often depending on their genotype, and the reproduction of the established protocols is not always possible. The purpose of this paper ...

Abstract Orchids with their sheer variety of species are amazing, a major sharer in global floriculture trade. Papilionanthe Miss Joaquim, well known hybrid orchid, also recognized as official National flower Singapore is resilient, sun loving which blossoms throughout the year. Though seed pod derived from single contains millions dusty seeds, due to lack endosperm, seeds cannot germinate with...

Cryopreservation of medicinal plants is an important tool for restoration of their potential to synthesize valuable secondary metabolites. Establishment of an efficient protocol requires detailed understanding of species/genotype-specific responses to changing external variables. The aim of this work was to study the genotype-dependent response of Hypericum perforatum L. shoot tips to different...

Embryo rescue refers to an in vitro technology have been used for propagating plants under from poorly developed embryos by avoiding embryo abortion. The aim of the present study was regeneration a medicinally wild tree species Buchanania lanzan through method. Isolated immature dissected out de-coded seeds collected 15 years old trees were cultured Woody Plant Medium (WPM) test germination. 10...