background: babesia ovis and theileria ovis are among the important and main etiological agents causing ovine babesiosis and ovine theileriosis, causing severe economic losses among sheep and goats. the aim of the present study was to determine the prevalence and molecular diagnosis of b. ovis and t. ovis in lohi sheep at livestock experiment station bahadurnagar, okara, pakistan. methods: the ...

Scorpion venom is the richest source of peptide toxins with high levels of specific interactions with different ion-channel membrane proteins. The present study involved the amplification and sequencing of a 310-bp cDNA fragment encoding a beta-like neurotoxin active on sodium ion-channel from the venom glands of scorpion Androctonus crassicauda belonging to the Buthidae family using r...

a polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) test was performed to investigate the allele frequencies of the insulin-like growth factor (igf1 and igf2 genes) in 90 broilerchickens. a 793 bp fragment of igf1 gene and 1146 bp fragment of igf2 gene were amplified and digested with hinfi and hsp92ii restriction enzymes,respectively. two types of alleles of a and b...

a highly efficient cloning vector was constructed for cloning pcr products by inserting an 80 bp dna fragment into pgem-5zf (+) vector. the xcm i digestion of this vector gave rise to a 3’ overhanging deoxythymidine offering the possibility of cloning pcr products with 3' adenosine overhang created by taq dna polymerase. furthermore, two ecor i sites were added to the construct for identificati...

seth4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of arabidopsis thaliana. this fragment was pcr amplified using kod hi fi dna polymerase enzyme. this fragment was cloned into pgbkt7 bate vector and transformed e. coli dh5? cells containing vector were selected on lb medium containing kanamycin. finally, pgbkt7-seth4 bate was transformed into yeast ...

Toxoplasmosis is a common and widespread infection in humans and many other species of warm–blooded animals. Toxoplasma gondii-derived heat shock protein 70 (Hsp70) may play an important role in the virulence of Toxoplasma gondii (T. gondii). In the present study, T. gondii Hsp70 was amplified by polymerase chain reaction (PCR) from the DNA of the T. gondiitachyzoite RH strain through the use o...

NBS-LRR-type disease resistance gene-like cDNA, induced by salicylic acid (SA) was cloned from rye Secalecereale L. (2n = 14RR) var. Petkus, which has rust genes such as Lr26, Sr31 andRr9. We designed primers based on the NBS region and performed PCR using Petkus genomic DNA a template. Next, we TA-cloned 532-bp fragment containing five homologous amino sequences in region. The SA-treated showe...

Background: Tuberculosis (TB) remains as a major cause of death around the world. Construction of a new vaccine against tuberculosis is an effective way to control it. Several vaccines against this disease have been developed. The aim of the present study was to cloning of tb10.4 gene in pcDNA3.1+ plasmid and evaluation of its expression in eukaryotic cells. ...