An HPLC method for determination of αand γ-tocopherol in rat blood has been optimized and validated. The optimum means of separation and analysis of tocopherol homologs were selected on the basis of procedures found in the literature; documentary evidence is presented which shows precision and accuracy were satisfactory. Spectrofluorimetric detection was performed after separation on a Separon ...

α-tocopherol is a vitamin E isoform with potent antioxidant activity, while the γ-tocopherol of exerts more pro-inflammatory effects. In maternal–fetal environments, increased plasma concentrations are associated positive birth outcomes, higher linked negative pregnancy outcomes. However, little known about tocopherol in placental tissue and their role modulating oxidative stress, process that ...

Tocopherols are essential components of the human diet and are synthesized exclusively by photosynthetic organisms. These lipophilic antioxidants consist of a chromanol ring and a 15-carbon tail derived from homogentisate (HGA) and phytyl diphosphate, respectively. Condensation of HGA and phytyl diphosphate, the committed step in tocopherol biosynthesis, is catalyzed by HGA phytyltransferase (H...

Background: Improvement of a-tocopherol content is an important breeding aim to increase the nutritional value of crops. Several efforts have been conducted to improve the a-tocopherol content in soybean [Glycine max (L.) Merr.] through transgenic technology by overexpressing genes related to a-tocopherol biosynthesis or through changes to crop management practices. Varieties with high a-tocoph...

Alpha-tocopherol is one of four tocopherol isoforms and has the highest vitamin E activity in humans. Most cultivated soybean seeds contain γ-tocopherol as the predominant form, and the ratio of α-tocopherol content to total tocopherol content (α-tocopherol ratio) is <10%. Three soybean accessions from Eastern Europe have α-tocopherol ratios of >20%. This higher content is likely due to mutatio...

Background: Selenium and -tocopherol, the major form of vitamin E in supplements, appear to have a protective effect against prostate cancer. However, little attention has been paid to the possible role of -tocopherol, a major component of vitamin E in the U.S. diet and the second most common tocopherol in human serum. A nested case–control study was conducted to examine the associations of -to...

Plasma and lipoprotein alpha-tocopherol concentrations of four patients with familial isolated vitamin E deficiency and six control subjects were observed for 4 d after an oral dose (approximately 15 mg) of RRR-alpha-tocopheryl acetate labeled with six deuterium atoms (d6-tocopherol). Chylomicron d6-tocopherol concentrations were similar in the two groups. d6-Tocopherol concentrations of plasma...

Designing enriched probiotic supplements may have some advantages including protection of probiotic microorganism from oxidative destruction, improving enzyme activity of the gastrointestinal tract and probably increasing half-life of micronutrient. In this study Saccharomyces cerevisiae enriched with α-tocopherol produced as an accumulator and transporter of a lipid soluble vitamin for the fir...

Coenzyme Q10(CoQ10) in human milk at different stages of maturity in mothers of preterm and full-term infants and its relation to the total antioxidant capacity of milk is described for the first time. Thirty healthy breastfeeding women provided colostrum, transition-milk and mature-milk samples. Coenzyme Q, alpha-, gamma- and delta-tocopherol, fatty acids and the total antioxidant capacity of ...

background programmed cell death (apoptosis) is a physiological process needed to remove unwanted or damaged cells. it has been hypothesized that any failure of programmed cell death leads to the development of neoplasm. identifying new agents which induce apoptosis in tumor cells is of great significance in treatment of neo­plasms. numerous studies suggest that exposure of tumor cells to stati...