PURPOSE Topical steroids are frequently used to control corneal inflammation and uveitis or is administered after surgery, to prevent corneal graft rejection. This study was undertaken to determine whether steroids could affect the pathogenicity of Acanthamoeba castellanii. METHODS The effect of dexamethasone phosphate on excystment, proliferation, and encystment of trophozoites and cysts of ...

PURPOSE To produce animal models of Acanthamoeba keratitis and to evaluate the advantages and adaptation range of each of the three methods employed. MATERIALS AND METHODS Mice and Wistar rats in three groups of 15 rats and 15 mice each were used to establish the models. Right corneas in group A were scratched and challenged with Acanthamoeba. Those in group B were scratched and covered with ...

BACKGROUND & OBJECTIVES Acanthamoeba keratitis (AK) is a painful and vision-threatening ocular infection. The differentiation of Acanthamoeba at the species and subspecies level is complicated. Nearly all the AK isolates have been shown to belong to T4 genotype when analysed by ribosomal RNA gene sequences and there is no universally acceptable method for differentiation of different subtypes o...

Background & Objectives: Cold Plasma is an emerging non-thermal, chemical-free, environmentally friendly disinfection technology. Plasma-activated water has received considerable attention from researchers in recent years. Despite extensive studies on the antibacterial effects of plasma-activated water, its anti-eukaryotic have not been identified. In humans, Acanthamoeba causes granulomatous e...

purpose: to report a case of bilateral acanthamoeba keratitis with recurrence after keratoplasties case report: here we report a case with bilateral acanthamoeba keratitis who initially responds to medical treatment, after discontinuation of treatment the disease flared up and not responded to medical therapy so superficial keratectomy was done and medical treatment continued and the ulcer impr...

acanthamoeba keratitis is a vision- threatening infection and the most important risk factors are contact lenses characteristic symptoms include disproportionately severe ocular pain, epithelial ulcer, ring stromal infiltrate and resistance to antimicrobial treatment. the aim is to report the first cases of acanthamoeba keratitis in yazd in a retrospective study confirmed on histopathological a...

Purpose: To report a case of bilateral acanthamoeba keratitis with recurrence after keratoplasties Case report: Here we report a case with bilateral acanthamoeba keratitis who initially responds to medical treatment, after discontinuation of treatment the disease flared up and not responded to medical therapy so superficial keratectomy was done and medical treatment continued and the ulcer impr...

Acanthamoeba castellanii is a free-living protozoan pathogen capable of causing a blinding keratitis and fatal granulomatous encephalitis. Current treatment generally involves an hourly application of polyhexamethylene biguanide (PHMB) over a period of several days but this is not entirely effective against all strains/isolates. The tolerance mechanisms of PHMB in Acanthamoeba cells remain uncl...

BACKGROUND Free-living amoeba (FLA)-related keratitis is a progressive infection of the cornea with poor prognosis. The present study aimed to investigates the contact lenses of patients with keratitis for pathogenic free-living amoebae. METHODS Overall, 62 contact lenses and their paraphernalia of patients with keratitis cultured and tested for the presence of free-living amoebae using morph...

Acanthamoeba keratitis is a vision-threatening infection caused by pathogenic species of the genus Acanthamoeba. Although not all Acanthamoeba spp. can cause keratitis, it is important to differentiate pathogenic species and isolates from nonpathogens. Since extracellular proteases may play a role in ocular pathology, we used colorimetric, cytopathic, and zymographic assays to assess extracellu...