نتایج جستجو برای: acridine orange and sperm chromatin
تعداد نتایج: 16844855 فیلتر نتایج به سال:
Background: Diabetes mellitus (DM), primary or idiopathic, is a chronic disorder of the carbohydrate, lipid and protein metabolism, characterized through hyperglycemia and glycosuria. DM can involve male reproductive function at many levels. Vitamin C ( ascorbic acid) is one of necessary elements in human and mammalian diet that is very important in fertility. Vitamin C prevent from gamets dama...
Introduction: Para-nonylphenol (p-NP) is an important environmental pollutant that can affect the male reproductive system through endocrine disruption and inducing of oxidative stressence. Hence, this study aimed to investigate the protective effect of Nigella sativa oil (NSO) on testicular tissue and sperm parameters (count, motility, viability and morphology), sperm DNA and chromatin abnorma...
OBJECTIVE The aim of the present study was to evaluate in 111 ejaculates from fertile men membrane integrity of spermatozoa before selection and sperm motility, and sperm concentration and chromatin integrity before and after selection of motile spermatozoo. METHODS We evaluated the membrane integrity (using hypoosmotic swelling test and Eosin-Y) before separation and chromatin integrity (usi...
BACKGROUND Reducing the percentage of sperm anomalies in insemination samples remains a goal to be achieved in the intra-cytoplasmic sperm insemination (ICSI) procedure. The aim of this study was to evaluate the efficiency of density gradient centrifugation (DGC) and Zeta methods to recover sperm with intact chromatin, and to evaluate whether the combined DGC/Zeta procedure improved ICSI outcom...
OBJECTIVE To test whether sperm with abnormal head morphology are more likely to undergo DNA damage and/or chromatin modification during the process of freeze-thawing. DESIGN In this prospective study, the semen samples from forty-four men attending the infertility clinic were included. Samples were divided into aliquots to allow direct comparison of fresh and frozen spermatozoa from the same...
The present report was designed to study the macrophage role and their association with percentage of spermatozoa showing DNA native, morphologically normal sperm and occurrence of antibody-coated spermatozoa. Human sperm samples from 31 patients were used in this study. All samples were evaluated for standard semen parameters according to World Health Organization criteria. The sperm heads wit...
INTRODUCTION: Micronuclei (MN) are microscopically visible round or oval cytoplasmic chromatin masses in the extra nuclear vicinity, originating from aberrant mitosis, chromatic fragments or whole chromosomes. The local factors like smoking exerts an influence on oral mucosa cells which are reflected as cellular changes resulting in the formation of MN. AIM: To evaluate and compare the MN index...
BACKGROUND Evaluating the significance and the effects of plant-derived drugs on laboratory animal's fertility was recognized. There was antioxidant activity reported from Heracleum persicum (Golpar). OBJECTIVE Current study aims to study the antioxidant effect of Golpar extracts on sperm parameters and chromatin quality in mice. MATERIALS AND METHODS Eighteen adult male mice were divided t...
Sperm chromatin integrity is vital for successful pregnancy and transmission of genetic material to the offspring. We evaluated chromatin integrity in sperm from 60 infertile men and 7 fertile donors comparing the sperm chromatin structure assay (SCSA), TdT-mediated-dUTP nick end labeling (TUNEL), the sperm chromatin dispersion (SCD) test, and acridine orange staining technique (AOT). The TUNEL...
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